LipoFluor-ER1™ Endoplasmic Reticulum Tracing Reagent

Name: LipoFluor-ER1™ Endoplasmic Reticulum Tracing Reagent
Brand: Biosensis
SKU: TR-601-ER1
Price: 297.0 USD
Availability: InStock

Comparison of TR-601-ER1 with ER-Tracker (upper panel). TR-601-ER1 can be used to stain the endoplasmic reticulum in live and fixed cells (lower panel).

Staining of endoplasmic reticulum in cultured cells.

Comparison of TR-601-ER1 with ER-Tracker (upper panel). TR-601-ER1 can be used to stain the endoplasmic reticulum in live and fixed cells (lower panel).

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Species Reactivity

All Mammals, Human, Mouse

Applications

ICC, IF

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Data Sheet

SKU: TR-601-ER1

Volume/Size: 4 vials x 5.5 µL per vial

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Product Details

Product Description

LipoFluor-ER1™ is a cell-permeant stain for the endoplasmic reticulum. LipoFluor-ER1™ passively diffuses across the plasma membrane into the cell, stains at low concentrations and has minimal cytotoxic effects. It can be used as a real-time imaging reagent which can be imaged within minutes of addition and has minimal photobleaching. LipoFluor-ER1™ is easily washed from cells, and therefore is ideal for protocols which require intermittent monitoring of endoplasmic reticulum structures. This tracing reagent can be used on live and fixed samples and is compatible with other dyes. LipoFluor-ER1™ has a large Stokes Shift, shows excellent resistance to photobleaching and demonstrates low cytotoxicity.

Target

Endoplasmic Reticulum Lipids

Immunogen Species

Human

Specificity

Fluorescent imaging of the endoplasmic reticulum compartment. Cell penetration and localisation of LipoFluor-ER1™ has been confirmed in a range of cell lines, including prostate cells (PNT2, PNT1a, LNCaP, 22RV1 and DU145), cardiomyocytes (H9c2) and neuronal cells (PC-12).

Species Reactivity

All Mammals, Human, Mouse

Applications

ICC, IF

Application Details

Fluorescent imaging of the endoplasmic reticulum compartment.Sample types: live cells, fixed cells (4% paraformaldehyde), and tissue (frozen only). Microscopy: Epifluorescence microscopy: YES, Confocal microscopy: YES, Multiphoton imaging: YES, Infrared spectroscopy: NO, Raman spectroscopy: NO; Visualization: Ex/Em: 405 nm / 570 nm. The typical working concentration is 50µM (1:1000 dilution) in an appropriate buffer or cell culture medium. Biosensis recommends that the end user determine optimal dilutions/concentrations.

Excitation / Emission

405 nm / 570 nm

Method of Analysis

Fluorescence

Sample Type

Cells, Tissues (live or fixed)

Buffer

DMSO

Physical State

Liquid, ready to dilute

Production Notes

Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of fluorescent isomers. No amount of starting material was detected.

Synonyms

LipoFluor-ER1™

Usage Statement

For research use only.

Country of Origin

United States

Expiration

If protected from light, the liquid 10 mM stock solution vials are stable for 12 months from date of receipt, at 2-8ºC. A vial should be diluted immediately prior to use and should be used within 24 hours for best results.

Storage

The liquid stock solution vials can be stored up to 12 months from date of receipt at 2-8°C, properly sealed and protected from light. Working solutions should be used within 24 hours and not stored for later use.

Shipping

25°C (ambient)

LipoFluor-ER1™ Protocol

Four 0.5 mL microfuge vials containing 5.5 uL/vial of 50 mM stock solution of Lipofluor™ TR-601-ER1 in DMSO. 164.1 μg per vial.

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LipoFluor-ER1™ Endoplasmic Reticulum Tracing Reagent

SKU: TR-601-ER1

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