LipoFluor-DMN-DT™ Lipid Droplets & ER Positive Vesicle Tracing Reagent
LipoFluor-DMN-DT™ is a new fluorescent imaging agent that can discern both LDs (lipid droplets) and ER (endoplasmic reticulum) compartments using a single excitation wavelength.
LipoFluor-DMN-DT™ is a new fluorescent imaging agent that can discern both LDs (lipid droplets) and ER (endoplasmic reticulum) compartments using a single excitation wavelength. DMN-DT can track the biogenesis of LDs and their subsequent dynamic movements, both within cells for a range of cell types and between adjacent cells. These actions can be visualized in real time given both regions of the cell emit at different wavelengths after treatment with LipoFluor-DMN-DT™. LipoFluor-DMN-DT™ is non-toxic with only a low concentration (10 µM) required for confocal imaging experiments.
Lipid Droplets & Endoplasmic Reticulum
Human
LipoFluor-DMN-DT ™ is a new fluorescent imaging agent that can discern both LDs (lipid droplets) and ER (endoplasmic reticulum) compartments using a single excitation wavelength.
All Mammals, Human, Mouse, Rat
ICC, IF
Each vial can be diluted 1000X depending upon the final working concentration of the dye in the experiment. Typical working concentrations for Lipofluor™ TR-607-DT are 10 μM to a maximum of 100 μM. This concentration was suitable for live cell imaging. It yielded high-quality images of lipid droplets with no evidence of toxicity, demonstrating the efficacy of DMN-DT at this concentration for visualizing lipid droplets in cellular contexts.
Fluorescence
Live cell culture and fixed brain tissue, other samples not yet tested
DMSO
Liquid, ready to dilute
Live cell culture and fixed brain tissue. Validated Cell lines: LipoFluor-DMN-DT ™ to stain multiple cell lines, a range of malignant (22Rv1, LNCaP, PC3, and HCT-8) and non-malignant (PNT1a and PNT2) cell types. Other cell lines are expected to stain as well when alive.
If protected from light, the liquid 10 mM stock solution vials are stable for 12 months from date of receipt, at 2-8°C. A vial should be diluted immediately prior to use and should be used within 24 hours for best results.
The liquid stock solution vials can be stored up to 12 months from date of receipt at 2-8°C, properly sealed and protected from light. Working solutions should be used within 24 hours and not stored for later use.
Four 0.5 mL microfuge vials containing 5.5 uL/vial of 10 mM stock solution of Lipofluor™ TR-607-DT in DMSO.
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