L-selectin-Fc Chimera, Human, Purified Recombinant Protein

Name: L-selectin-Fc Chimera, Human, Purified Recombinant Protein
Brand: Biosensis
SKU: PE-1247-50
Price: 527.0 USD
Availability: InStock

L-selectin-Fc Chimera, purified human recombinant protein, suitable to block.

Gel analysis of L-selectin-Fc Chimera.

Lane 1: MW marker
Lane 2: L-selectin-Fc Chimera (10 µg)
Lane 3: L-selectin-Fc Chimera (10 µg) treated with PNGase F to remove potential N-linked glycans

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Applications

Block

Host Species

HEK293

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Data Sheet

SKU: PE-1247-50

Volume/Size: 50 µg

Variant

Price:

$527.00

Quantity:

Product Details

Target

L-selectin

Scientific Background

L-selection (CD26L) is a cell surface adhesion protein constitutively expressed on all classes of human leukocytes. The L-Selectin glycoprotein is composed of a C-type lectin family domain at the amino terminus, an EGF-like domain, two short consensus repeat (SCR) sequences, a transmembrane domain and a short cytoplasmic tail. L-Selectin contains 7 potential N- linked glycosylation sites.

Format

Purified. The L-Selectin-Fc chimera consists of 20-45% carbohydrate by weight.

Applications

Block

Host Species

HEK293

Protein Sequence

A DNA sequence encoding the signal peptide, pro-peptide and extracellular domain of human L-Selectin (amino acids 1-343) was fused to the Fc region of human IgG1 (amino acids 93-330). The chimeric protein was expressed in modified human 293 cells.

Storage

Reconstitute with sterile-filtered phosphate-buffered saline to a concentration of 100 µg/mL. Lyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

Physical State

Lyophilized

Production Notes

>95% purity, as determined by SDS-PAGE and visualized by silver stain

Buffer

When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Lymph node homing receptor; Leukocyte adhesion molecule 1; LAM-1; Leukocyte surface antigen Leu-8; TQ1; gp90-MEL; Leukocyte-endothelial cell adhesion molecule 1; LECAM1; CD62L; SELL; LNHR; LYAM1

UniProt ID

P14151

Shipping

25°C (ambient)

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L-selectin-Fc Chimera, Human, Purified Recombinant Protein

SKU: PE-1247-50

Product Details

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