Human Oligomeric Amyloid-beta ELISA Assay
The oligomeric form of Amyloid Beta peptide (Aβ, 1-42) has been closely linked to Alzheimer's Disease. The Biosensis oligomeric Aβ ELISA kit is a sandwich ELISA that allows the preferential quantification of oligomeric Aβ peptides. This kit is exclusive to Biosensis and consists of a pre-coated mouse monoclonal anti-Aβ capture antibody (MOAB-2), a biotinylated MOAB-2 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin.
This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.
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| Reagent | 1 Plate Kit | 2 Plate Kit |
|---|---|---|
| Capture antibody coated 96 well microplate | 12 × 8 wells | 24 × 8 wells |
| Assay diluent B (1x) | 2 × 25 mL | 4 × 25 mL |
| Pre-formed, oligomerized Aβ42 standard | 2 × 10 ng | 4 × 10 ng |
| Oligomerized Aβ42 positive control (Lyophilized, see vial label for amount) | 2 vials | 4 vials |
| Aβ detection antibody (100x) | 1 × 110 μL | 2 × 110 μL |
| Streptavidin-HRP (100x) | 1 × 110 μL | 2 × 110 μL |
| Wash buffer (10x) | 1 × 33 mL | 2 × 33 mL |
| TMB substrate (1x) | 1 × 11 mL | 2 × 11 mL |
| TMB stop solution (1x) | 1 × 11 mL | 2 × 11 mL |
| Plate sealer | Supplied | |