Human NT-4/5 Rapid ELISA Assay
The Biosensis Neurotrophin 4/5 Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT4/5 in less than 4 hours in cell culture supernatants, human citrate-plasma and brain extracts. This NT4/5 ELISA kit employs a recombinant human NT4/5 standard. The capture and detection antibodies will also detect NT4/5 from other species due to a high degree of NT4/5 amino acid sequence homology. Therefore, this ELISA kit can be used to quantify NT4/5 in many species including mouse, rat and monkey.
This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.
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| Reagent | 1 Plate Kit | 2 Plate Kit |
|---|---|---|
| NT4/5 antibody coated 96 well microplate | 12 × 8 wells | 24 × 8 wells | Assay diluent A (1X) | 2 × 25 mL | 4 × 25 mL |
| Recombinant human 4/5 standard | 2 × 2 ng | 4 × 2 ng | Quality Control (QC) Sample | 2 Vials (see vial label for amount) | 4 Vials (see vial label for amount) |
| NT4/5 detection antibody (100X) | 1 × 110 µL | 2 × 110 µL | Streptavidin-HRP (100X) | 1 × 110 µL | 2 × 110 µL |
| Wash buffer (10X) | 1 × 33 mL | 2 × 33 mL | TMB substrate (1X) | 1 × 11 mL | 2 × 11 mL |
| TMB stop solution (1X) | 1 × 11 mL | 2 × 11 mL | Plate sealer | Supplied |