Human LR3 insulin-like Growth Factor-I (LR³IGF-1) was developed by GroPep Bioreagents (www.gropep.com) specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR³IGF-1 at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR³IGF-1 is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules, which are responsible for promoting cell survival by inhibition of apoptosis.

This LR³IGF-1 Rapid ELISA kit combines GroPep's many years of expertise in the field of IGF research and Biosensis' newly established Rapid ELISA platform. This collaboration has resulted in the new LR³IGF-1 Rapid ELISA kit, which provides for the sensitive, specific and reliable quantification of LR³IGF-1 protein in less than 3 hours!

The ELISA kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR³IGF-1 in culture media. Included in the kit are mouse monoclonal anti- LR³IGF-1 capture antibody pre-coated onto an ELISA plate, standard LR³IGF-1 protein, a biotinylated anti- LR³IGF-1 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of LR³IGF-1 present in samples and the supplied protein standard.

This LR³IGF-1 Rapid ELISA kit has been developed, optimized and validated to quantify LR³IGF-1 protein in cell culture medium. It is likely to be used to measure LR³IGF-1 in media and during downstream processing of media following a production cycle and is not intended for other use. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

LR³IGF-1 is an 83 amino acid analogue of IGF-I comprising the complete human IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus.

LR³IGF-1

Recombinant LR3-IGF1 protein, made in E.coli

Human

3.9 ng/mL - 200 ng/mL

Typical limit of detection (LOD) for LR_IGF-I is 1 ng/mL determined as 150% of the blank value.

Human. The assay is intended for quantification of LR³IGF-1. Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.

Human

ELISA

Culture Supernatant

Human LR3 insulin-like Growth Factor-I.

P05019 (unmodified)

For research use only.

United States

12 months from purchase.

Store at 2-8°C

Shipped on ice packs