Human IL1-RA Rapid ELISA Assay
The Biosensis Human IL-1RA Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of interleukin-1 receptor antagonist (IL-1RA) in less than 4 hours in human saliva only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Parallelism of four human saliva samples, serially diluted.
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| Reagent | 1 Plate Kit | 2 Plate Kit |
|---|---|---|
| Human IL1-RA Antibody Coated 96 well microplate | 1 plate | 2 plates |
| Assay Diluent H, 1X | 25 mL x 2 | 25 mL x 4 |
| Quality Control (QC) Sample | 2 Vials | 4 Vials |
| Recombinant Human IL1-RA Standard | 960 pg x 2 | 960 pg x 4 |
| Streptavidin-HRP, 100X | 110 μL x 1 | 110 μL x 2 |
| IL1-RA Detection Antibody | 43 μL x 1 | 43 μL x 2 |
| Wash Buffer, 10X | 33 mL x 1 | 33 mL x 2 |
| TMB Substrate, 1X | 11 mL x 1 | 11 mL x 2 |
| Stop Solution, 1X | 11 mL x 1 | 11 mL x 2 |
| Plate sealer | Supplied | |