Human a-synuclein Rapid ELISA Assay

Name: Human a-synuclein Rapid ELISA Assay
Brand: Biosensis
SKU: BEK-2216-2P
Price: 787.0 USD
Availability: InStock

This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.

Parallelism of calibration curve and diluted citrate-plasma samples from healthy individuals demonstrates accurate quantification of alpha-synuclein in human citrate-plasma.

Accurate quantification of alpha-synuclein in human serum from healthy individuals across four dilutions.

This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.

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Species Reactivity

Human, Mouse, Rat

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Data Sheet

SKU: BEK-2216-2P

Volume/Size: 2 Plate Kit

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Product Description

The Biosensis Alpha-Synuclein Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of alpha-synuclein in less than 4 hours in human citrate-plasma, serum, CSF, as well as mouse and rat brain homogenates only if used as directed. Please refer to the kit protocol for specific use instructions for blood and CSF application. This ELISA kit consists of a pre-coated sheep polyclonal anti-alpha-synuclein (aa: 116-131) capture antibody, a biotinylated mouse monoclonal anti-alpha-synuclein detection antibody (aa: 61-95) and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of alpha-synuclein present in samples and protein standards. A human alpha-synuclein positive control (QC sample) is provided to assure consistent assay performance. This ELISA kit has not been tested for other substrate applications, only citrate plasma and CSF, other substrates have not been tested and performance may vary. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.

Scientific Background

Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. Alpha synuclein has been associated with several neurodegenerative diseases. A point mutation in the gene coding for the alpha-synuclein protein was the first discovery linking this protein to a rare familial form of Parkinson's disease (PD). Subsequently, other mutations in the alpha-synuclein gene have been identified in familial PD. The aggregated proteinaceous inclusions called Lewy bodies found in PD and cortical Lewy body dementia (LBD) were discovered to be predominantly alpha-synuclein. Aberrant aggregation of alpha-synuclein has been detected in an increasing number of neurodegenerative diseases, collectively known as synucleopathies. Alpha-synuclein exists physiologically in both soluble and membrane-bound states, in unstructured and alpha-helical conformations, respectively. The physiological function of alpha-synuclein appears to require its translocation between these subcellular compartments and interconversion between the 2 conformations. Abnormal processing of alpha-synuclein is predicted to lead to pathological changes in its binding properties and function.

Target

Alpha-synuclein

Immunogen

The alpha-synuclein ELISA kit employs a recombinant human standard expressed in E.coli.

Immunogen Species

Human

Range

0.16 - 10 ng/mL

Sensitivity

Typical limit of detection (LOD) for alpha-synuclein is < 100 pg/mL, determined as alpha-synuclein concentration at blank OD plus 3x standard deviation of blank OD (n=10).

Specificity

Human, mouse and rat alpha-synuclein. Interference and cross-reactivity of human beta- and gamma-synuclein was assessed by spiking each protein at excess concentration of 20 ng/mL into two human plasma samples, each diluted 1:10 and 1:20, in comparison to unspiked samples. Cross-reactivity was calculated based on increase or decrease of apparent α-synuclein concentrations in spiked samples. At a 3-6 fold excess (w/v) of spiked protein over endogenous alpha-synuclein at both sample dilutions, this ELISA shows very little cross-reactivity of 2.3% or less.
Individual alpha-synuclein isoforms have not yet been tested, but it is expected that this ELISA kit detects monomeric, oligomeric and phosphorylated alpha-synuclein isoforms.

Species Reactivity

Human, Mouse, Rat

Method of Analysis

Colorimetric

Sample Type

CSF, Plasma (Citrate), Serum, Tissue Homogenates

Synonyms

Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP; SNCA; PARK1

UniProt ID

P37840

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months from purchase.

Storage

Store at 2-8°C

Shipping

Shipped on ice packs

Human a-synuclein Rapid ELISA Assay Protocol
Technical Note 1 - ELISA Validation
Technical Note 2 - ELISA Data Analysis
Calculation of Antigen Amount in Tissues and Lysates

Reagent	1 Plate Kit	2 Plate Kit
α-synuclein antibody coated 96 well microplate	12 × 8 wells	24 × 8 wells
Sample Diluent A (1X)	1 × 33 mL	2 × 33 mL
Assay diluent A (1X)	2 × 25 mL	4 × 25 mL
Recombinant human α-synuclein standard	2 × 20 ng	4 × 20 ng
Quality Control (QC) Sample	2 Vials (see vial label for amount)	4 Vials (see vial label for amount)
α-synuclein detection antibody (100X)	1 × 110 µL	2 × 110 µL
Streptavidin-HRP (100X)	1 × 110 µL	2 × 110 µL
Wash buffer (10X)	1 × 33 mL	2 × 33 mL
TMB substrate (1X)	1 × 11 mL	2 × 11 mL
TMB stop solution (1X)	1 × 11 mL	2 × 11 mL
Plate sealer	Supplied

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Human a-synuclein Rapid ELISA Assay

SKU: BEK-2216-2P

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