Anti-Aldolase C Antibody (4A9)

Name: Anti-Aldolase C Antibody
Brand: Biosensis
SKU: M-1693-100
Price: 327.0 USD
Availability: InStock

Our Anti-Aldolase C mouse monoclonal primary antibody detects bovine, human, mouse, pig, and rat Aldolase C, and is IgG. It is validated for use in ICC, WB.

Crude bovine cerebellum homogenate. The antibody recognizes the 40 kDa protein.

Mixed neuron/glial cultures stained with M-1693-100 (green) reveals strong cytoplasmic staining in astrocytes and our rabbit antibody to NeuN/FOX3 (R-3770-100) which shows nuclear and distal cytoplasmic staining in neuron cells and is completely absent in the astrocytes The blue stain reveals DNA in the nuclei of these cells.

Left (A): Rat cerebellum section stained by Immunohistochemistry with mouse antibody to aldolase C (green) at 1:1,000 dilution. Section was co-stained with rabbit antibody to GFAP (R-1374-50, red, 1:5,000). Blue: Hoechst nuclear stain. The aldolase C antibody selectively labels the perikarya and dendrites of Purkinje cells, while the GFAP antibody stains processes of Bergman glia and astrocytes. Left (B) Analysis of aldolase C expression (green) in E20 rat cortical culture by Immunocytochemistry. Cells were stained with mouse antibody to aldolase C (green, 1:1,000), and co-stained with chicken antibody to MAP2 (C-1382-50, red, 1:10,000). Blue: Hoechst nuclear stain. The aldolase C antibody labels cytosolic protein expressed in glial cells, while MAP2 antibody stains dendrites and perikarya of mature neurons. Right: Western blot analysis of aldolase C expression (green) in tissue lysates using mouse antibody to aldolase C (1:2,000). [1] protein standard, [2] rat brain, [3] mouse brain, [4] cow cerebellum, and [5] pig hippocampus. The single strong band at about 40 kDa corresponds to the aldolase C protein.

Crude bovine cerebellum homogenate. The antibody recognizes the 40 kDa protein.

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Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Applications

ICC, WB

Host Species

Mouse

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Data Sheet

SKU: M-1693-100

Volume/Size: 100 µg

Variant

Price:

$327.00

Quantity:

Product Details

Target

Aldolase C

Scientific Background

Aldolases are glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose 1,6-bisphosphate and fructose-1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde 3-phosphate or glyceraldehyde, respectively. Three aldolase isozymes are found in mammals specifically aldolases A, B, and C, each of which is encoded by a separate gene. Aldolase A is generally considered to be a muscle enzyme. Northern analysis of cultured cells suggests that it is present in both neurons and glia (1). Aldolase B is considered to be a liver-specific enzyme and it is transcriptionally activated by signals from hormones and dietary factors (2). In the adult, aldolase C is the brain-specific isozyme, with low but detectable activity in fetal tissues (1, 3-6). Aldolase C shares 81% amino acid identity with aldolase A and 70% identity with aldolase B. Earlier studies using isozyme-specific antibodies report its location in gray matter astrocytes and cells of the pia mater (5, 8). In situ hybridization of mouse central nervous system using isozyme-specific probes revealed that aldolase A and C are expressed in complementary cell types: aldolase A mRNA is found in neurons; aldolase C message is detected in astrocytes, some cells of the pia mater, and Purkinje cells (9). Aldolase C can in some situations be used as an astrocyte marker. However Purkinje cells of the cerebellum contain high levels of the enzyme, so the enzyme is not totally astrocyte specific.

Format

IgG

Clonality

Monoclonal

Clone

4A9

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Molecular Weight

40 kDa

Immunogen

N-terminal 20 amino acids of aldolase C protein, MPHSYPALSAEQKKELSDIA

Immunogen Species

Human

Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Production Notes

Protein G purified

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Dilution Ranges

WB: 1:1000-1:2000
ICC: 1:500-1:1000

Conjugate

Unconjugated

Specificity

The antibody reacts with a 40 kDa band by Western blot on a crude bovine cerebellum homogenate. It has also been used successfully for immunocytochemistry.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Brain-type aldolase, Fructose-bisphosphate aldolase C

UniProt ID

P09972

Shipping

25°C (ambient)

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Anti-Aldolase C Antibody (4A9)

SKU: M-1693-100

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