Anti-Agouti related protein (AGRP) Antibody

Our Anti-Agouti related protein (AGRP) guinea pig polyclonal primary antibody detects human, mouse, rat, and sheep Agouti related protein (AGRP), and is whole serum. It is validated for use in IHC-Frozen.

The 4% PFA fixed, frozen cryostat section of the sheep hypothalamus was incubated in guinea pig polyclonal antibodies to the mouse AGRP protein at the dilution of 1:1000 overnight followed by incubation with biotinylated secondary antibodies. Cell bodies and nerve terminals in the sheep brain are intensely stained. This figure shows staining of cells when no pre-absorption is performed.
Immunohistochemical detection of AGRP in the arcuate nucleus of rat brain using Guinea pig polyclonal antibody to mouse agouti related protein (GP-029-50) incubated at the dilution of 1:1000-2000 in PBS overnight followed by incubation with Alexa-568 goat anti-guinea pig (1:500) secondary antibody. Section is 4% PFA fixed, frozen, cyrosectioned material. In rodents nerve terminals are more often identified than whole cell bodies using GP-029-50
Immunohistochemical detection of agouti-related protein (AGRP) synthesizing neurons in the human infundibular nucleus using the GP-029-50 primary antibody (1:50 000), followed by its visualization with the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.
Immunohistochemical detection of agouti-related protein (AGRP) synthesizing neurons in the arcuate nucleus of the rat using the GP-029-50 primary antiserum (1:50 000), followed by its visualization with the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.
The 4% PFA fixed, frozen cryostat section of the sheep hypothalamus was incubated in guinea pig polyclonal antibodies to the mouse AGRP protein at the dilution of 1:1000 overnight followed by incubation with biotinylated secondary antibodies. Cell bodies and nerve terminals in the sheep brain are intensely stained. This figure shows staining of cells when no pre-absorption is performed.
The 4% PFA fixed, frozen cryostat section of the sheep hypothalamus was incubated in guinea pig polyclonal antibodies to the mouse AGRP protein at the dilution of 1:1000 overnight followed by incubation with biotinylated secondary antibodies. Cell bodies and nerve terminals in the sheep brain are intensely stained. This figure shows staining of cells when no pre-absorption is performed.
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Human, Mouse, Rat, Sheep
IHC
Guinea Pig

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Data Sheet

SKU: GP-029-50

Volume/Size: 50 µL
Price:
$337.00

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