Products: Primary Antibodies

Anti-Kvbeta2 K+ channel (K17/70)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-021	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-021	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat Zebrafish

Applications:

IHC IP WB

Immunogen:

Fusion protein amino acids 1-367 (full length) of rat Kv2 (accession number NP_034728)Epitope mapped to within amino acids 17-22

Human: 98% identity (362/367)
Mouse: 100% identity

Cross Reactivity:

None Reported

Protein Name(s):

Voltage-gated potassium channel subunit beta-2 (EC 1.1.1.-) (K(+) channel subunit beta-2) (Kv-beta-2) (Neuroimmune protein F5)

Gene Name(s):

Kcnab2 Ckbeta2 I2rf5 Kcnb3

Antibody Registry ID:

Purified: AB_2131373
TC Supernatant: AB_10673520

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

38 kDa (major band)41 kDa (minor band)

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Condro MC, White SA.. (2014), 'Distribution of language-related Cntnap2 protein in neural circuits critical for vocal learning. .' Journal of Comparative Neurology. 10.1002/cne.23394.
Kole MJ, Qian J, Waase MP, Klassen TL, Chen TT, Augustine GJ, Noebels JL.. (2015), 'Selective Loss of Presynaptic Potassium Channel Clusters at the Cerebellar Basket Cell Terminal Pinceau in Adam11 Mutants Reveals Their Role in Ephaptic Control of Purkinje Cell Firing. .' Journal of Neuroscience. 10.1523/JNEUROSCI.1346-15.2015.
Bavassano C, Marvaldi L, Langeslag M, Sarg B, Lindner H, Klimaschewski L, Kress M, Ferrer-Montiel A, Knaus HG.. (2013), 'Identification of voltage-gated K(+) channel beta 2 (Kvβ2) subunit as a novel interaction partner of the pain transducer Transient Receptor Potential Vanilloid 1 channel (TRPV1). .' Biochimica et Biophysica Acta. 10.1016/j.bbamcr.2013.09.001.
Ovsepian SV, Steuber V, Le Berre M, O'Hara L, O'Leary VB, Dolly JO.. (), 'A defined heteromeric KV1 channel stabilizes the intrinsic pacemaking and regulates the output of deep cerebellar nuclear neurons to thalamic targets. .' The Journal of Physiology. 10.1113/jphysiol.2012.249706.
Williams MR, Fuchs JR, Green JT, Morielli AD.. (2012), 'Cellular mechanisms and behavioral consequences of Kv1.2 regulation in the rat cerebellum..' Journal of Neuroscience. 10.1523/JNEUROSCI.6504-11.2012.
Tipparaju SM, Li XP, Kilfoil PJ, Xue B, Uversky VN, Bhatnagar A, Barski OA.. (2012), 'Interactions between the C-terminus of Kv1.5 and Kvβ regulate pyridine nucleotide-dependent changes in channel gating. .' Pflügers Archiv: European Journal of Physiology. 10.1007/s00424-012-1093-z.
Duflocq A, Chareyre F, Giovannini M, Couraud F, Davenne M.. (2011), 'Characterization of the axon initial segment (AIS) of motor neurons and identification of a para-AIS and a juxtapara-AIS, organized by protein 4.1B..' BMC Biology. 10.1186/1741-7007-9-66.
Gu C, Gu Y.. (2011), 'Clustering and activity tuning of Kv1 channels in myelinated hippocampal axons..' The Journal of Biological Chemistry. 10.1074/jbc.M111.219113.
Ohnishi H, Murata T, Kusakari S, Hayashi Y, Takao K, Maruyama T, Ago Y, Koda K, Jin FJ, Okawa K, Oldenborg PA, Okazawa H, Murata Y, Furuya N, Matsuda T, Miyakawa T, Matozaki T.. (2010), 'Stress-evoked tyrosine phosphorylation of signal regulatory protein α regulates behavioral immobility in the forced swim test. .' Journal of Neuroscience. 10.1523/JNEUROSCI.0257-10.2010.
Stapels M, Piper C, Yang T, Li M, Stowell C, Xiong ZG, Saugstad J, Simon RP, Geromanos S, Langridge J, Lan JQ, Zhou A.. (2010), 'Polycomb group proteins as epigenetic mediators of neuroprotection in ischemic tolerance. .' Science Signaling. 10.1126/scisignal.2000502.
Pineda RH, Knoeckel CS, Taylor AD, Estrada-Bernal A, Ribera AB.. (2008), 'Kv1 potassium channel complexes in vivo require Kvbeta2 subunits in dorsal spinal neurons..' Journal of Neurophysiology. 10.1152/jn.90667.2008.
Ogawa Y, Horresh I, Trimmer JS, Bredt DS, Peles E, Rasband MN.. (2008), 'Postsynaptic density-93 clusters Kv1 channels at axon initial segments independently of Caspr2. .' Journal of Neuroscience. 10.1523/JNEUROSCI.4431-07.2008.
Wenzel HJ, Vacher H, Clark E, Trimmer JS, Lee AL, Sapolsky RM, Tempel BL, Schwartzkroin PA.. (2007), 'Structural consequences of Kcna1 gene deletion and transfer in the mouse hippocampus. .' Epilepsia. 10.1111/j.1528-1167.2007.01189.x.
Gu C, Zhou W, Puthenveedu MA, Xu M, Jan YN, Jan LY.. (2006), 'The microtubule plus-end tracking protein EB1 is required for Kv1 voltage-gated K+ channel axonal targeting. .' Neuron. 10.1016/j.neuron.2006.10.022.