Products: Primary Antibodies

Anti-Kvbeta2 K+ channel Antibody (K17/70)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-021	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-021	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat Zebrafish

Applications:

IHC IP WB

Target Description:

Voltage-gated potassium channel subunit beta-2 or Kvß2 is encoded by the gene KCNAB2. Kvß2 is a member of the potassium channel, voltage-gated, shaker-related subfamily. Kvß2 is a cytoplasmic subunit that modulates the characteristics of the membrane spanning, channel-forming alpha-subunits. Beta subunits can promote the closure of channels or enhance channel activity depending on other channel proteins. Kvß2 is expressed in brain, in thecerebellum and in the juxtaparanodal region of nodes of Ranvier. Kvß2 expression is also detected in lung, heart and kidney. Diseases associated with KCNAB2 include Chromosome 1P36 Deletion Syndrome and Juvenile Amyotrophic Lateral Sclerosis 16,

Immunogen:

Fusion protein amino acids 1-367 (full length) of rat Kv2 (accession number NP_034728)Epitope mapped to within amino acids 17-22; Human: 98% identity (362/367); Mouse: 100% identity

Cross Reactivity:

No cross-reactivity reported

Protein Name(s):

Kvbeta2 K+ channel

Gene Name(s):

Kcnab2 Ckbeta2 I2rf5 Kcnb3

Antibody Registry ID:

Purified: AB_2131373
TC Supernatant: AB_10673520

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

38 kDa (major band)41 kDa (minor band)

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.