Polyclonal Antibodies

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Western blot of β-Catenin immunoprecipitated from A431 cells treated with pervanadate. The blots were probed with β-Catenin (Tyr-86) or β-Catenin (a.a. 649-661) antibodies. The immunoprecipitates were untreated (lanes 1 & 6) or treated with alkaline phosphatase (lanes 5 & 10), and the antibodies were incubated with β-Catenin (Tyr-86) (lanes 2,7) or β-Catenin (a.a. 649-661) (lanes 3,8) peptides or phospho-Tyr (lanes 4,9).Immunocytochemical labeling of β-Catenin in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal β-Catenin (CM1181) or rabbit polyclonal β-Catenin (Tyr-86) or β-Catenin (Y654) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-β-Catenin (Tyr-86), Phosphospecific Antibody
$340
Western blot analysis of Hct116 src transformed cells (20 µg/lane) serum starved overnight or treated with pervanadate (1 mM) for 30 min. The blot was probed with anti-β-Catenin or anti-β-Catenin (Tyr-142)Formalin fixed, citric acid treated parafin sections of embryonic Rat E16 intestines. Sections were probed with anti-β-Catenin (CP1061) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
PhosphoSolutions Anti-β-Catenin (N-terminal) Antibody
$285
Western blot analysis of rat PC12 cells treated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then the blot was treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-α-Catenin monoclonal (lanes 1 & 2), anti-α1-Catenin (Tyr-148) phospho-specific (lanes 3 & 4), or anti-α1-Catenin (a.a. 143-153) (lanes 5 & 6).
PhosphoSolutions Anti-α1-Catenin (Tyr-148), Phosphospecific Antibody
$340
Western blot analysis of rat PC12 cells treated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then the blot was treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-α-Catenin monoclonal (lanes 1 & 2), anti-α1-Catenin (Tyr-148) phospho-specific (lanes 3 & 4), or anti-α1-Catenin (a.a. 143-153) (lanes 5 & 6).
PhosphoSolutions Anti-α1-Catenin (N-terminal region) Antibody
$285
Western blot image of human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. then the blots were untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-VE-cadherin (Tyr-685) (lanes 1 & 2) or mouse monoclonal anti-VE-cadherin (lanes 3 & 4).Phosphorylation of CDH5 Y658, CDH5 Y685 (Cat. CP1981) and VEGFR2 (Tyr1054/1059) in cultured human LECs after Yoda1 (0.3 µM) treatment for the indicated periods. Image from publication CC-BY-4.0. PMID: 38528202
PhosphoSolutions Anti-VE-Cadherin (Tyr-685), Phosphospecific Antibody
$340
Immunocytochemical labeling of VE-Cadherin in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with rabbit polyclonal VE-Cadherin (a.a. 770-781), then the antibody was detected using appropriate secondary antibody conjugated to Cy3. Phase image (left) and fluorescent image (right).
PhosphoSolutions Anti-VE-Cadherin (C-terminal) Antibody
$285
Western blot image of human endothelial cells untreated or treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-N-Cadherin (Cytoplasmic), anti-N-Cadherin (Tyr-820), anti-N-Cadherin (a.a. 853-864), and anti-N-Cadherin (Tyr-860).Immunocytochemical labeling of phosphorylated N-Cadherin in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal N-Cadherin (Cytoplasmic) and rabbit polyclonal N-Cadherin(Tyr-860) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-N-Cadherin (Y860) [E-Cadherin (Y835)], Phosphospecific Antibody
$340
Western blot image of human endothelial cells untreated or treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-N-Cadherin (Cytoplasmic), anti-N-Cadherin (Tyr-820), anti-N-Cadherin (a.a. 853-864), and anti-N-Cadherin (Tyr-860).
PhosphoSolutions Anti-N-Cadherin (C-terminal region) Antibody
$285
Western blot image of human endothelial cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blots were probed with anti-N-cadherin (a.a. 811-824) (lanes 1 & 2) and anti-unphosphorylated N-cadherin (Tyr-820) (lanes 3 & 4).
PhosphoSolutions Anti-Unphosphorylated N-Cadherin (Tyr-820) Antibody
$340
Western blot image of human endothelial cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2, 4, 5 & 6). The blots were probed with anti-N-Cadherin (Cytoplasmic) (lanes 1 & 2) and anti-N-cadherin (Tyr-820) (lanes 3-6). The latter antibody was used in the presence of no peptide (lane 4), phospho-N-cadherin (Tyr-820) peptide (lane 5), or phospho-N-cadherin (Tyr-860) peptide (lane 6).Immunocytochemical labeling of phosphorylated N-Cadherin in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal N-Cadherin (Cytoplasmic) and rabbit polyclonal N-Cadherin(Tyr-820) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-N-Cadherin (Tyr-820), Phosphospecific Antibody
$340
Western blot image of human endothelial cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blots were probed with anti-N-cadherin (a.a. 811-824) (lanes 1 & 2) and anti-unphosphorylated N-cadherin (Tyr-820) (lanes 3 & 4).Western blot image of mouse brain lysate immunoprecipitated with no antibody (lane 1), anti-N-Cadherin (CP1751) rabbit polyclonal antibody (lane 2), and whole mouse brain lysate (lane 3). The blot was probed with anti-N-cadherin (Cytoplasmic) mouse monoclonal antibody (lanes 1-3) and detected using anti-Mouse Ig Light Chain specific:HRP secondary antibody.
PhosphoSolutions Anti-N-Cadherin (C-terminal region) Antibody
$285
Western blot image of human A431 cells that were probed with rabbit polyclonal anti-E-Cadherin (a.a. 774-786) at 1:250 (lane 1), 1:1000 (lane 2), and 1:4000 (lane 3) or mouse monoclonal anti-E-cadherin (Cytoplasmic) at 1:250 (lane 4) and 1:1000 (lane 5).
PhosphoSolutions Anti-E-Cadherin (C-terminal region) Antibody
$285
Western blot of 293 cells mock transfected (lane 1) or transiently transfected with pLenti6/TR lentiviral vector (lanes 2 & 3). Blots were probed with anti-Bsd (lanes 1-3). Molecular weight (MW) standards show that Bsd is expressed as a 13 kDa band in only the transfected cells.Immunocytochemical labeling of Blasticidin S Deaminase (Bsd) in 293 cells mock transfected (left) and transiently transfected with pLenti6/TR lentiviral vector (right). The cells were labeled with anti-Bsd (BP1231) and detected using appropriate secondary antibody conjugated to Texas Red. (Images provided by Charles Mashburn and Dr. George Smith at the University of Kentucky, Spinal Cord and Brain Injury Research Center).
PhosphoSolutions Anti-Blasticidin S Deaminase Antibody
$340
Western blot analysis of mouse J774A.1 macrophage stimulated with calyculin A (lanes 1-4) then dephosphorylated with lambda phosphatase (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Bad (lanes 1 & 2), and rabbit polyclonal anti-Bad (Ser-112) (lanes 3 & 4).
PhosphoSolutions Anti-Bad (Ser-112), Phosphospecific Antibody
$340
Western blot analysis of mouse J774A.1 macrophage stimulated with calyculin A (lanes 1-4) then dephosphorylated with lambda phosphatase (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Bad (lanes 1 & 2), and rabbit polyclonal anti-Bad (Ser-26) (lanes 3 & 4).
PhosphoSolutions Anti-Bad (Ser-26), Phosphospecific Antibody
$340
Immunocytochemical labeling of ATM phosphorylation in calyculin A-treated A431 cells. The cells were labeled with rabbit polyclonal anti-ATM (Ser-794) (AP3631) antibody in the absence (Left) or presence (Right) of blocking peptide (AX3635). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-ATM (Ser-794), Phosphospecific Antibody
$340
Western blot analysis of Arpc1b phosphorylation in human A431 stimulated with calyculin A (100 nM) for 30 min (lanes 1 & 3). The blots were treated with lambda phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Arpc1b (C-terminal region) (lanes 1 & 2) or anti-Arpc1b (Thr-21) (lanes 3 & 4).
PhosphoSolutions Anti-Arpc1b/p41-Arc (Thr-21), Phosphospecific Antibody
$340
Western blot of rat A7r5 (lane 1), human Jurkat (lane 2), rat PC12 (lane 3), human PC3 (lane 4), mouse C2C12 (lane 5), and human A431 cells (lane 6). The blot was probed with rabbit polyclonal anti-Arpc1b (C-terminal region).
PhosphoSolutions Anti-Arpc1b/p41-Arc (C-terminal region) Antibody
$340
Western blot of human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells. The blots were probed with rabbit polyclonal anti-Arp3 (C-terminal region) antibody at 1:1000 (lanes 1-3).Immunocytochemical labeling of Arp3 in aldehyde-fixed and NP-40-permeabilized rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-Arp3 (C-terminal region) (AP4581) in the absence (left) or presence (right) of blocking peptide (AX4585). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-Arp3 (C-terminal region) Antibody
$285
Western blot of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with anti-Arp2 (C-terminal) (lanes 1 & 2) or anti-Arp2 (Thr-237/Thr-238) (lanes 3 & 4).Immunocytochemical labeling of Arp2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with Arp2 (C-terminal region) and Arp2 (Thr-237/Thr-238) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to Cy3.
PhosphoSolutions Anti-Arp2 (Thr-237/Thr-238), Phosphospecific Antibody
$340
Western blot analysis of human A431 cells treated with EGF (100 ng/ml for 60 min.) (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rat monoclonal anti-Ago2 (AM5271) (lanes 1 & 2) and rabbit polyclonal anti-Ago2 (Tyr-393) phospho-specific antibody (lanes 3 & 4).
PhosphoSolutions Anti-Argonaute 2 (Tyr-393), Phosphospecific Antibody
$340
Western blot analysis of mouse recombinant Ago2 full length protein (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonal anti-Ago2 (AP5281) (lanes 1 & 2) and rabbit polyclonal anti-Ago2 (Ser-387) phospho-specific antibody (lanes 3 & 4).
PhosphoSolutions Anti-Argonaute 2 (Ser-387), Phosphospecific Antibody
$340
Western blot analysis of mouse recombinant Ago2 full length protein (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonal anti-Ago2 (AP5281) (lanes 1 & 2) and rabbit polyclonal anti-Ago2 (Ser-387) phospho-specific antibody (lanes 3 & 4).
PhosphoSolutions Anti-Argonaute 2 Antibody
$285
Western blot analysis of A431 cells, serum starved overnight (20 µg/lanes 1 & 3) and calyculin A (100 nM) treated for 30 minutes (20 µg/lanes 2 & 4). The blot was probed with anti-Akt (Thr-34) (lanes 1 & 2) or anti-Akt1 (N-terminal region) (lanes 3 & 4).
PhosphoSolutions Anti-Akt (Thr-34), Phosphospecific Antibody
$340

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