Polyclonal Antibodies

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Western blot analysis of α-actinin in human A431 cells (lanes 1 & 3) and rabbit spleen fibroblasts (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-α-actinin 4 (a.a. 2-11) or mouse monoclonal anti-α-actinin (C-terminal region).
PhosphoSolutions Anti-α-Actinin 4 (N-terminal) Antibody
$285
Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide (AX1655) or phospho-Actin (Tyr-53) peptide (AX1675), respectively (right).
PhosphoSolutions Anti-Actin (Tyr-53), Phosphospecific Antibody
$340
Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide (AX1655) or phospho-Actin (Tyr-53) peptide (AX1675), respectively (right).
PhosphoSolutions Anti-Actin (N-terminal region) Antibody
$285
Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (AP1271; lanes 3 & 4), or anti-c-Abl (Tyr-245) (AP1251; lanes 5 & 6).Representative ABL1p-Y412 (cat. AP1271) immunohistochemistry of tumors or para-carcinom in human breat cancer tissue are shown. Image from publication CC-BY-4.0. PMID: 39060421
PhosphoSolutions Anti-c-Abl (Tyr-412), Phosphospecific Antibody
$340
Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (AP1271; lanes 3 & 4), or anti-c-Abl (Tyr-245) (AP1251; lanes 5 & 6).
PhosphoSolutions Anti-c-Abl (Tyr-245), Phosphospecific Antibody
$340
Immunofluorescence of healthy human kidney. The antibody specifically labels pre-pro-vasopressin (white) in the collecting ducts (DBA, red), but not in the proximal tubules (LTL, green) of human kidney. DNA is labeled with DAPI (blue). Image kindly provided by JP Arroyo, Vanderbilt University.Western blot of mouse whole brain lysate showing specific immunolabeling of the ~20 kDa uncleaved pre-pro vasopressin protein.
PhosphoSolutions Anti-Pre-Pro-Vasopressin Antibody
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Immunostaining of a sagittal section of FFPE rat brain showing strong staining of oligodendrocyte precursor cells (OPC) in the hippocampus using our anti-NG2/CSPG4 (DAB, 1:200) and detected with anti-rabbit HRP. The section is counterstained with hematoxylin to identify DNA (blue).Western blot of mouse whole brain lysate showing specific immunolabeling of the ~250 kDa CSPG4/NG2 protein.
PhosphoSolutions Anti-NG2/CSPG4 Antibody
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Immunofluorescence of transfected COS-7 cells specifically labeling positive beta Catenin cells using our anti-beta Catenin antibody (1:200, red) and anti-FLAG antibody (green). The yellow color results from the overlap of cells labeled with both antibodies. DNA is labeled with DAPI (blue).Western blot of rat whole brain lysate showing specific immunolabeling of the ~92 kDa beta Catenin protein.
PhosphoSolutions Anti-beta Catenin Antibody
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Immunostaining of mouse striatum showing specific labeling of CNP in myelinating oligodendrocytes (Cat. # 326-CNP, green, 1:3000) and neurofilament L (red, 1:5000). The blue is staining DNA. Western blot of rat hippocampal lysate showing the specific immunolabeling of the ~46 kDa CNP protein.
PhosphoSolutions Anti-CNP (2,3-cyclic nucleotide-3-phosphodiesterase) Antibody
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Immunostaining of a section of rat olfactory bulb showing specific labeling of MeCP2 in neuronal nuclei (Cat. No. 1207-MECP2, red, 1:2000) and alpha synuclein (green). The blue is DAPI staining of nuclear DNA.Western blot of mouse whole brain lysate showing specific immunolabeling of the MeCP2 protein at ~75 kDa.
PhosphoSolutions Anti-MeCP2 Antibody
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Western blot of mouse whole brain lysate showing specific labeling of the ~130 kDa IRE1 protein phosphorylated at Ser724 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is eliminated by blot treatment with lambda phosphatase (1200 units for 30 minutes).
PhosphoSolutions Anti-IRE1 (Ser724) Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~120 kDa TAO2 phosphorylated at Ser181 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-TAO2 (Ser181) Antibody
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Western blot of mouse heart lysate showing specific immunolabeling of the ~250 kDa and ~150 kDa splice variants of the XIRP1 protein phosphorylated at Ser295 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-Xin Actin-binding Repeat-containing Protein 1 (XIRP1) (Ser295) Antibody
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Western blot of mouse testicular lysate showing specific immunolabeling of the ~104 kDa PTPH1 phosphorylated at Ser459 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase , 400 units/100ul lysate for 30 minutes, RT).
PhosphoSolutions Anti-PTPH1 (Ser459) Antibody
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Western blot of Drosophila lysate showing specific immunolabeling of the ~60 kDa PRAS40 protein phosphorylated at Thr356 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-PRAS40 (Thr356) Antibody
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Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 kDa PLK protein phosphorylated at Thr210 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-Polo-Like Kinase (Thr210) Antibody
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Western blot of rat hippocampal membrane lysate showing specific immunolabeling of the ~120 kDa Polo-Like Kinase Kinase phosphorylated at Ser482,486,490 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units overnight).
PhosphoSolutions Anti-Polo-Like Kinase Kinase (Ser482,486,490) Antibody
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Western blot of HEK293 cells transfected with Parkin wild type (WT) and Parkin S378 mutant(Mut) showing the specific immunolabeling of the ~52 kDa parkin protein phosphorylated at Ser378.
PhosphoSolutions Anti-Parkin (Ser378) Antibody
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Western blot of HEK293 cells transfected with Parkin wild type (WT) and Parkin S101 mutant (Mut) showing the specific immunolabeling of the ~52 kDa parkin protein phosphorylated at Ser101.
PhosphoSolutions Anti-Parkin (Ser101) Antibody
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Western blot of Jurkat cell lysate showing specific immunolabeling of the ~48 kDa p62 phosphorylated at Ser28 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-p62 (Ser28) Antibody
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Western blot of mouse whole brain lysate showing specific labeling of the ~86 kDa PanK4 phosphorylated at Ser63 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is nearly eliminated with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-PanK4 (Thr63) Antibody
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Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~68 kDa PAK1 protein phosphorylated at Thr84 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase , 800 units/1mg protein for 30 minutes).
PhosphoSolutions Anti-PAK-1 (Thr84) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68 kDa to ~70 kDa PAK protein phosphorylated at Ser402 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-PAK-1,2,3 (Thr402) Antibody
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Western blot of Jurkat cell lysate showing specific immunolabeling of the ~28 kDa rpS6 phosphorylated at Ser244 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-Ribosomal Protein S6 (Ser244) Antibody
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