Anti-Arp2 (Thr-237/Thr-238), Phosphospecific Antibody

Name: Anti-Arp2 (Thr-237/Thr-238), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: AP3871
Price: 340.0 USD
Availability: InStock

4 Citations

Our Arp2 (Thr-237/Thr-238) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Arp2 (Thr-237/Thr-238) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with anti-Arp2 (C-terminal) (lanes 1 & 2) or anti-Arp2 (Thr-237/Thr-238) (lanes 3 & 4).

Immunocytochemical labeling of Arp2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with Arp2 (C-terminal region) and Arp2 (Thr-237/Thr-238) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to Cy3.

Western blot of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with anti-Arp2 (C-terminal) (lanes 1 & 2) or anti-Arp2 (Thr-237/Thr-238) (lanes 3 & 4).

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Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: AP3871

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Arp2 (Thr-237/Thr-238)

Scientific Background

Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. Arp2 has two phosphosites, Thr-237 and Thr-238, that are evolutionarily conserved, and are phosphorylated along with Tyr-202 in response to growth factor stimulation. These phosphorylation events may regulate binding to the pointed end of actin filaments, and alanine substitutions of these Arp2 phosphosites inhibit membrane protrusions. Thus, phosphorylation may be another mode of Arp2/3 complex regulation in addition to the activity of nucleation-promoting factors.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

ACTR2

Molecular Weight

Immunogen

Phospho-Arp2 (Thr-237/Thr-238) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding threonine 237 and threonine 238 in human Arp2. This peptide sequence is highly conserved in rat, mouse, chicken, and fish Arp2 proteins.

Immunogen Species

Human

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects 44 and 32 kDa* proteins corresponding to the molecular mass of Arp2 on SDS-PAGE immunoblots of human A431 and Jurkat cells treated with Calyculin A. This reactivity is not observed after lambda phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

T237/T238

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

ACTR2, Arp2

UniProt ID

P61160

Shipping

Blue Ice

Product Specific References for Applications and Species

Immunohistochemistry: Lancelet
Western Blot: Mouse | Rat

Immunohistochemistry: Lancelet
PMID	Dilution	Publication
27307516	1:100	Morov, AR, et al. 2016. Acquisition of the dorsal structures in chordate amphioxus. Open Biology, 160062.

Western Blot: Mouse
PMID	Dilution	Publication
35951202	not listed	Alhadidi, QM, et al. 2022. MiR-182 Inhibition Protects Against Experimental Stroke in vivo and Mitigates Astrocyte Injury and Inflammation in vitro via Modulation of Cortactin Activity. Neurochemical Research, 3682-3696.
33664738	not listed	Jati, S, et al. 2021. Wnt5A-Mediated Actin Organization Regulates Host Response to Bacterial Pathogens and Non-Pathogens. Frontiers in immunology, 628191.

Western Blot: Rat
PMID	Dilution	Publication
27073215	1:1000	Lauterborn, J, et al. 2017. Cofilin Activation Is Temporally Associated with the Cessation of Growth in the Developing Hippocampus. Cerebral cortex (New York, N.Y. : 1991), 2640-2651.

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Anti-Arp2 (Thr-237/Thr-238), Phosphospecific Antibody

SKU: AP3871

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Arp2 (Thr-237/Thr-238), Phosphospecific Antibody

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