Polyclonal Antibodies

797 products

Polyclonal antibodies recognize multiple epitopes on the same target protein and therefore can offer strong signal-to-noise as the binding at multiple sites allows for many detection molecules to bind.

Antibodies Inc is proud to offer several product lines and flavors of polyclonal antibodies. Rabbit polyclonals are an industry standard for polyclonal antibodies because the medium size of rabbits allows for larger pools of serum. With a focus in both neuroscience and cancer research, our PhosphoSolutions line specializes in phosphospecific rabbit polyclonal antibodies.

Chicken polyclonals have grown in popularity especially when performing multiplex analysis using secondary antibodies. We offer the complete product line from Aves Labs: a pioneer in the use of chickens for the production of polyclonal research antibodies. Evolutionarily removed from mammals, chickens mount a vigorous immune response to mammalian gene products, resulting in high-affinity IgY antibodies. A unique species like chicken is also perfect for multiplexing applications, with low cross-reactivity to other species.

Antibodies Inc offers polyclonal centromere antibodies which are purified IgG derived from human anti-centromere positive serum (CREST patient serum). Useful as diagnostic markers in assays such as IHC and ICC, as well as controls in ANA diagnostics, they help identify autoimmune reactions.

Showing 577 - 600 of 797 products

Western blot image of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1, 3, & 5) or treated with lambda phosphatase (lanes 2, 4, & 6) then were probed with anti-EGFR (a.a. 961-972) (lanes 1 & 2), anti-EGFR (Ser-967) (lanes 3 & 4), and anti-EGFR (Ser-1142) (lanes 5 & 6).

Immunocytochemical labeling in A431 cells untreated or treated with Calyculin A (50 nM) for 15 min. Cells were labeled with anti-EGFR (a.a. 961-972) or anti-EGFR (Ser-1142) antibodies. The specificity of labeling was demonstrated for each antibody by blocking the signals with their respective blocking peptides, EGFR (a.a. 961-972) (EX1875) and phospho-EGFR (Ser-1142) (EX1935).

PhosphoSolutions Anti-EGFR (Ser-1142), Phosphospecific Antibody

$340

PhosphoSolutions Anti-EGFR (N-terminal region) Antibody

$285

Recombinant human eEF2K untreated (lanes 1 & 4), auto-phosphorylated in the presence of Ca2+ and Calmodulin (lanes 2 & 5), and dephosphorylated with lambda phosphatase (lanes 3 & 6). The blots were probed with rabbit polyclonal anti-eEF2K (N-terminus) (lanes 1-3) or anti-eEF2K (Ser-500) (lanes 4-6). (Images provided by the laboratory of Dr. Kevin Dalby in the Dept. of Pharmacy at the University of Texas at Austin.)

Western blot image of C2C12 untreated (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min. (lanes 2 & 4). The blot was probed with anti-eEF2K (N-terminus) (lanes 1 & 2) and anti-eEF2K (Ser-500) (lanes 3 & 4).

PhosphoSolutions Anti-eEF2K (Ser-500), Phosphospecific Antibody

$340

Western blot image of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1-6), then some lanes of the blot were treated with lambda phosphatase (lanes 2, 4 & 6). The blot was probed with rabbit polyclonals anti-eEF2K (C-terminus) (lanes 1 & 2), anti-eEF2K (Ser-445) phospho-specific (lanes 3 & 4), and anti-eEF2K (Ser-398) phospho-specific (lanes 5 & 6).

PhosphoSolutions Anti-eEF2K (Ser-445), Phosphospecific Antibody

$340

PhosphoSolutions Anti-eEF2K (Ser-398), Phosphospecific Antibody

$340

Recombinant human eEF2K untreated (lanes 1 & 4) and auto-phosphorylated in the presence of Ca2+ and Calmodulin (lanes 2 & 5). In some lanes, the eEF2K was dephosphorylated with lambda phosphatase (lanes 3 & 6). The blots were probed with rabbit polyclonal anti-eEF2K (N-terminus) (lanes 1-3) or anti-eEF2K (Thr-348) (lanes 4-6). (Images provided by the laboratory of Dr. Kevin Dalby in the Dept. of Pharmacy at the University of Texas at Austin.)

PhosphoSolutions Anti-eEF2K (Thr-348), Phosphospecific Antibody

$340

Western blot image of C2C12 treated with calyculin A (100 nM) for 30 min. (lanes 1 & 3), then the blot was treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-eEF2K (N-terminus) at 1:500 (lanes 1 & 2) and anti-eEF2K (Ser-78) (EP4391) at 1:1000.

PhosphoSolutions Anti-eEF2K (Ser-78), Phosphospecific Antibody

$340

Western blot image of eEF2K in human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells, and immunoprecipitation of eEF2K from HeLa cell lysate using anti-eEF2K (C-terminus) antibody (lanes 4 & 6). Negative control immunopreciptations with no antibody are shown in lane 5 and 7. The blots were probed with anit-eEF2K (C-terminus) (lanes 1-5) or with anti-eEF2K (N-terminal) antibody (lanes 6 & 7).

Immunocytochemical labeling of eEF2K in paraformaldehyde fixed and NP-40 permeabilized A431 cells. The cells were labeled with rabbit polyclonal anti-eEF2K (EP4661). The antibody was detected using goat anti-rabbit DyLight® 594.

PhosphoSolutions Anti-eEF2K (C-terminus) Antibody

$285

Western blot analysis of adult mouse brain untreated (lanes 1, 3, 5, & 7) or treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with rat monoclonal anti-EB3 (EM5091) (lanes 1 & 2), and rabbit polyclonals anti-EB3 (Ser-162) (lanes 3 & 4), anti-EB3 (Ser-176) (lanes 5 & 6), and anti-EB3 (a.a. 171-182) (lanes 7 & 8).

PhosphoSolutions Anti-EB3 (Ser-176), Phosphospecific Antibody

$340

PhosphoSolutions Anti-EB3 (Ser-162), Phosphospecific Antibody

$340

PhosphoSolutions Anti-EB3 (central region) Antibody

$285

Western blot analysis of draxin expression in rat PC12 cells (lane 1), rat P1 brain (lane 2), adult mouse brain (lane 3), and chick E9 brain (lane 4). The blot was probed with rabbit polyclonal anti-Draxin (C-terminal region) at 1:1000.

Immunocytochemical labeling of Draxin in rat PC12 cells differentiated with NGF. The cells were probed with Draxin (C-terminal region) rabbit polyclonal antibody, then the antibody was detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (DX3675). Lower images show corresponding phase images.

PhosphoSolutions Anti-Draxin (C-terminal region) Antibody

$285

Western blot image of Jurkat cells stimulated with calyculin A (100 nM, 30 min) (lanes 1-6) followed by lambda phosphatase (lanes 2 & 6) or alkaline phosphatase (lane 4) treatment. The blots were probed with anti-Dok1 (lanes 1 & 2), anti-Dok1 (Tyr-362) (lanes 3 & 4), and anti-Dok1 (Ser-450) (lanes 5 & 6).

PhosphoSolutions Anti-Dok1 (Ser-450), Phosphospecific Antibody

$340

PhosphoSolutions Anti-Dok1 (Tyr-362) [Dok2 (Tyr-337)], Phosphospecific Antibody

$340

Western blot of DISC1 phosphorylation in PC12 cells untreated (lanes 1 & 3) or treated with calyculin A (100 nm) for 30 min (lanes 2 & 4). The blots were probed with DP3041 anti-DISC1 (a.a. 740-753) (lanes 1 & 2) or DP3061 anti-DISC1 (Ser-710) (lanes 3 & 4).

PhosphoSolutions Anti-DISC1 (Ser-710), Phosphospecific Antibody

$340

Western blot of DISC1 in mouse brain (lanes 1 & 3) and rat PC12 cells (lanes 2 & 4). The blots were probed with DP3021 anti-DISC1 (a.a. 740-753) (lanes 1 & 2) and DP3041 anti-DISC1 (a.a. 740-753) (lanes 3 & 4).

PhosphoSolutions Anti-DISC1 Antibody

$285

PhosphoSolutions Anti-DISC1 (C-terminus) Antibody

$285

Western blot analysis of mDia3 expression in human Jurkat cells treated with Calyculin A (100 nM) (lanes 1-4). The blots were treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal anti-mDia3 (C-terminus; DP4511) (lanes 1 & 2) and anti-phospho-mDia3 (Ser-196; DP4521) (lanes 3 & 4).

PhosphoSolutions Anti-mDia3 (Ser-196)) [conserved site], Phosphospecific Antibody

$340

PhosphoSolutions Anti-mDia3 (C-terminus) Antibody

$340

Western blot analysis of COS-7 cells expressing flag-tagged mDia2 (lanes 1-3) or mDia2 mutant (T1061A) (lane 4). The cells were untreated (lane 1) or treated with calyculin A (lanes 2 & 3). The blot was treated with alkaline phosphatase (lane 2), then probed with anti-mDia2 (Thr-1061) (upper blot) or anti-flag antibody (lower blot). (Image provided by Dr. Christopher Mack in the Dept. of Pathology at UNC Chapel Hill).

PhosphoSolutions Anti-mDia2 (Thr-1061), Phosphospecific Antibody

$340

Western blot analysis of mDia2 expression in rat PC12 (lane 1), human A431 (lane 2), mouse brain (lane 3), and rabbit spleen fibroblasts (lane 4). The blots were probed with anti-mDia2 (C-terminal region) at 1:500.

Immunofluorescent images showing Parvalbumin (hair cells, red) and Diaph3 (Cat no DP3491, 1:400, magenta) expression in cryo-sectioned cochlea (organ of Corti area). The knockdown of the endogenous Stub1 in mouse inner ear leads to severe hearing loss. AAV-ie vector containing GFP tag was used to package Stub1-shRNA. The left ear of P3 mice were injected with AAV-ie via round window membrane (RWM). Image from publication, CC-BY-4.0. PMID: 39322015.

PhosphoSolutions Anti-mDia2 (C-terminal region) Antibody

$340

Western blot analysis of mDia1 expression in human Jurkat cells (lanes 1-4). The blots were probed with anti-mDia1 (a.a. 66-77: DP4471) in the presence (lane 1) or absence of blocking peptide (DX4475) using dilutions of 1:250 (lane 2), 1:1000 (lane 3), and 1:4000 (lane 4).

PhosphoSolutions Anti-mDia1 (N-terminal region) Antibody

$340

Western blot analysis of human Jurkat cells treated with 100 nM calyculin A for 30 min. then the blots were untreated (-) or treated (+) with lambda phosphatase. The blots were probed with rabbit polyclonals anti-CXCR4 (Ser-324/Ser-325) (left panel) or anti-CXCR4 (a.a. 322-329) (right panel).

Immunocytochemical labeling of CXCR4 in chick pluripotent cells. The cells were labeled with rabbit polyclonal CXCR4 (S324/S325) antibody (CP4251), then detected using appropriate secondary antibody (Red). (Image provided by Dr. Yangqing Lu at the Regenerative Bioscience Center, University of Georgia).

PhosphoSolutions Anti-CXCR4 (Ser-324/Ser-325), Phosphospecific Antibody

$340

Immunocytochemical labeling of CXCR4 in chick pluripotent cells. The cells were labeled with rabbit polyclonal CXCR4 (a.a. 328-338) antibody (CP4231), then detected using appropriate secondary antibody (Red). (Image provided by Dr. Yangqing Lu at the Regenerative Bioscience Center, University of Georgia).

PhosphoSolutions Anti-CXCR4 (C-terminal region) Antibody

$285

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Polyclonal Antibodies

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