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Immunofluoresence of COS7 cells expressing mCherry using chicken anti-mCherry antibody (green) and showing mCherry autofluorescence (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-mCherry signal and mCherry autofluoresence in transfected cells.IMCD3 cells transfected with Arl13b-mCherry​ and stained with Anti-mCherry Antibody (Cat. MCHERRY, 1:300) and visualized​ with A594 anti-chicken secondary antibody. ​ As expected, cytoplasmic and ciliary signals are observed in transfected cells.​ Image kindly provided by Svetlana Makova, Yale University.
Aves Labs Anti-mCherry Antibody
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Western Blot of transfected HEK 293T cell lysate showing specific immunolabeling of MCT1.
Western blot analysis of mDia1 expression in human Jurkat cells (lanes 1-4). The blots were probed with anti-mDia1 (a.a. 66-77: DP4471) in the presence (lane 1) or absence of blocking peptide (DX4475) using dilutions of 1:250 (lane 2), 1:1000 (lane 3), and 1:4000 (lane 4).
Western blot analysis of mDia2 expression in rat PC12 (lane 1), human A431 (lane 2), mouse brain (lane 3), and rabbit spleen fibroblasts (lane 4). The blots were probed with anti-mDia2 (C-terminal region) at 1:500.Immunofluorescent images showing Parvalbumin (hair cells, red) and Diaph3 (Cat. DP3491, 1:400, magenta) expression in cryo-sectioned cochlea (organ of Corti area). The knockdown of the endogenous Stub1 in mouse inner ear leads to severe hearing loss. AAV-ie vector containing GFP tag was used to package Stub1-shRNA. The left ear of P3 mice were injected with AAV-ie via round window membrane (RWM). Image from publication, CC-BY-4.0. PMID: 39322015.
Western blot analysis of mDia3 expression in human Jurkat cells treated with Calyculin A (100 nM) (lanes 1-4). The blots were treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal anti-mDia3 (C-terminus; DP4511) (lanes 1 & 2) and anti-phospho-mDia3 (Ser-196; DP4521) (lanes 3 & 4).
Western blot of adult mouse brain tissue lysate. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (Ser-80) (lanes 1 & 2) or anti-MeCP2 (C-terminus) (lanes 3 & 4).Immunocytochemical labeling of MeCP2 in rat PC12 cells differentiated with NGF. The cells were probed with MeCP2 (C-terminus) rabbit polyclonal antibody (MP4591) in the absence (left) or presence (right) of blocking peptide (MX4595). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
Adult rat hippocampus immunohistochemistry.Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N227/21 (left) and loading control (right) TC supe.

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