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Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-c-Cbl (CM1591; lanes 1 & 2) or anti-c-Cbl (Tyr-700) (CM1611; lanes 3 & 4).
Immunohistochemistry against hippocampus from adult rats treated with pilocarpine to induce seizures (top) and with saline (bottom).Immunoblot against crude brain low-speed pellets from untreated adult rats (RBLSP), adult rats treated with kainic acid to induce seizures (RBLSP seiz) and adult rats treated with saline (RBLSP non-seiz) probed with N486/32 (left), N408/62 (middle) and N52A/42 (right) TC supe.
Anti-c-FOS AntibodyAnti-c-FOS Antibody
Western blot. Left Lane -- Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. Lane 1, 30 µg protein; lane 2, 20 µg protein; lane 3, 10 µg protein; lane 4, 5 µg protein.
Western blot of GST fusion protein containing human C-Raf. The blot was probed with polyclonal anti-C-Raf (C-terminus) antibody in the presence (lanes 2-4) or absence (lane 1) of C-Raf (C-terminus) blocking peptide (lane 2), C-Raf (Ser-471) peptide (lane 3), or unrelated peptide (lane 4).
Western blot of human A431 (lane 1) and Jurkat (lane 2) cells probed with mouse monoclonal anti-C-Raf (N-terminal) antibody at 1:500.
Western blot analysis of mouse SYF cells transformed with c-Src then left untreated (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with anti-c-Src (Tyr-215) (lanes 1 & 2), anti-c-Src (N-terminal region) (lanes 3 & 4), and anti-c-Src (Tyr-530) (lanes 5 & 6).

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