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Adult rat hippocampus immunohistochemistry.Immunoblot versus lysates of COS cells transiently transfected with BKBeta3a or Kv2.1 plasmids, probed with N40B/18 TC supe.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with BKBeta4 or Kv2.1 plasmids and probed with L18A/3 pure IgG at 10 mg/mL (left panel) or K89/41 TC supe (right panel).
Western blot of 293 cells mock transfected (lane 1) or transiently transfected with pLenti6/TR lentiviral vector (lanes 2 & 3). Blots were probed with anti-Bsd (lanes 1-3). Molecular weight (MW) standards show that Bsd is expressed as a 13 kDa band in only the transfected cells.Immunocytochemical labeling of Blasticidin S Deaminase (Bsd) in 293 cells mock transfected (left) and transiently transfected with pLenti6/TR lentiviral vector (right). The cells were labeled with anti-Bsd (BP1231) and detected using appropriate secondary antibody conjugated to Texas Red. (Images provided by Charles Mashburn and Dr. George Smith at the University of Kentucky, Spinal Cord and Brain Injury Research Center).
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc- tagged Botch1 and untagged Kv2.1 plasmids and probed with N116/14 (left panel) and K89/34 (right panel) TC supe.Adult rat hippocampus immunohistochemistry.

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