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Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS-1 cells transfected with Ataxin- 1-WT, Ataxin-1-S751A or Kv2.1 plasmid and probed with N65/37 TC supe.
Adult rat brain immunohistochemistry.Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged ATF4 or untagged ATF5 or Kv2.1 plasmid and probed with N360A/24 TC supe (left), Rabbit anti-ATF5 (middle) or K89/34 TC supe (right).
Western blot of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1, 3, & 5) or treated with lambda phosphatase (lanes 2, 4, & 6) then probed with anti-ATM (Ser-794) (lanes 1 & 2), anti-ATM (C-Terminal) (lanes 3 & 4), or anti-ATM (Ser-1981) (lanes 5 & 6).Immunocytochemical labeling of ATM phosphorylation in control (Top row) or calyculin A-treated A431 cells (Bottom row). The cells were labeled with mouse monoclonal ATM (C-terminal region) (AM3611) and ATM (Ser-1981) (AM3661). The antibodies were detected using goat anti-mouse-DyLight® 594.

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