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Western blot image of human recombinant Atrogin 1 (lanes 1-6). The blot was probed with rabbit polyclonal Atrogin-1 (lanes 1-3) and rat monoclonal Atrogin-1 (lanes 4-6) at 1:1000 (lanes 1 & 4), 1:2000 (lanes 2 & 5), and 1:4000 (lanes 3 & 6).
Western blot image of mouse gastrocnemius (lanes 1 & 3) and mouse diaphragm tissue lysate (lanes 2 & 4). The blot was probed with anti-Atrogin-1 (AP2041; lanes 1-4) in the presence (lanes 3 & 4) or absence (lanes 1 & 2) of Atrogin-1 peptide (AX2045).Formalin fixed, citric acid treated paraffin sections of E16 mouse skeletal muscle. Sections were probed with anti-Atrogin-1 (AP2041) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
Western blot of human Jurkat cells treated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated (lanes 2 & 4) with lambda phosphatase and probed with anti-B-Raf (N-terminus) (lanes 1 & 2) or anti-B-Raf (Ser-446) (lanes 3 & 4).
Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged BAF53a, BAF53b or Kv2.1 plasmids and probed with N336B/83 (left) or N52A/42 (right) TC supe. Note that BAF53a is ubiquitously expressed in many different cell types, including COS cells.
Adult rat hippocampus immunohistochemistry.Immunoblot versus samples from adult rat brain (RBLSP) and WT and BAF53b KO mouse brains probed with N332B/15 (left) and N336B/83 (right) TC supes. Mouse samples courtesy of Ann Kuo and Gerald Crabtree (Stanford).
Array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L124/59 (magenta), rabbit mAb PSD-95 (Cell Signaling 3450, green) and Hoechst nuclear stain (blue). The insert shows three consecutive sections through the synapse that is marked with a white box.Adult rat hippocampus immunohistochemistry.
NeuroMab Anti-Bassoon Antibody
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