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Western blot analysis of human Jurkat cells treated with 100 nM calyculin A for 30 min. then the blots were untreated (-) or treated (+) with lambda phosphatase. The blots were probed with rabbit polyclonal anti-CXCR4 (Ser-324/Ser-325) (left panel), or anti-CXCR4 (a.a. 328-338) (right panel).Immunocytochemical labeling of CXCR4 in chick pluripotent cells. The cells were labeled with rabbit polyclonal CXCR4 (a.a. 328-338) antibody (CP4231), then detected using appropriate secondary antibody (Red). (Image provided by Dr. Yangqing Lu at the Regenerative Bioscience Center, University of Georgia).
Western Blot of transfected 293T cells showing specific immunolabeling of Cyclin E1.
Western Blot of CT10 cells showing specific immunolabeling of Cyclin E2.
Western blot analysis of human LNCaP (lane 1), MCF7 (lane 2), MDA-MB-231 (lane 3), and MeWo (lane 4) cell lysates, as well as human recombinant full-length cyclophilin B (lane 5) and cyclophilin A (lane 6). The blot was probed with mouse monoclonal anti-cyclophilin B (CM0191) at 1:500.Immunocytochemical labeling of cyclophilin B in methanol:acetone (1:1) fixed human A549 lung cancer cells. The cells were labeled with mouse monoclonal anti-cyclophilin B (CM0191). The antibody was detected using goat anti-mouse DyLight® 594.

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