Products: Primary Antibodies

Anti-LAR/PTPRF (Receptor-type tyrosine-protein phosphatase) (N165/38)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-193	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-193	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:

ICC WB

Immunogen:

Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR (also known as Leukocyte antigen related, Leukocyte common antigen-related phosphatase, Receptor-type tyrosine-protein phosphatase F and PTPRF, accession number P10586)
Rat: 97% identity (286/293 amino acids identical)
Mouse: 97% identity (285/293 amino acids identical)
>80% identity with PTPRD and PTPRS
>50% identity with PTPRM and PTPRK

Cross Reactivity:

Does not cross-react with PTPRD or PTPRS

Protein Name(s):

Receptor-type tyrosine-protein phosphatase F (EC 3.1.3.48) (Leukocyte common antigen related) (LAR)

Gene Name(s):

PTPRF LAR

Antibody Registry ID:

Purified: AB_10675291
TC Supernatant: AB_10673278

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

85 kDa(full-length is 210 kDa, smaller due to proteolysis into P-subunit containing transmembrane and intracellular domains)

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Song YS, Lee HJ, Prosselkov P, Itohara S, Kim E.. (2013), 'Trans-induced cis interaction in the tripartite NGL-1, netrin-G1 and LAR adhesion complex promotes development of excitatory synapses. .' Journal of Cell Science. 10.1242/jcs.129718.
van der Veer EP, de Bruin RG, Kraaijeveld AO, de Vries MR, Bot I, Pera T, Segers FM, Trompet S, van Gils JM, Roeten MK, Beckers CM, van Santbrink PJ, Janssen A, van Solingen C, Swildens J, de Boer HC, Peters EA, Bijkerk R, Rousch M, Doop M, Kuiper J, Schalij MJ, van der Wal AC, Richard S, van Berkel TJ, Pickering JG, Hiemstra PS, Goumans MJ, Rabelink TJ, de Vries AA, Quax PH, Jukema JW, Biessen EA, van Zonneveld AJ.. (2013), 'Quaking, an RNA-binding protein, is a critical regulator of vascular smooth muscle cell phenotype. .' Circulation Research. 10.1161/CIRCRESAHA.113.301302.