Array tomography immunofluorescence of Lowicryl-embedded sections from adult mouse cerebellum with L109/57 (red) and DAPI (blue). Left panel is a single 70 nm section imaged with a 20X objective, with a scale bar of 50 µm. Right panel is a MAX projection of 10 serial 70 nm sections imaged with a 63X objective, with a scale bar of 20 µm. Images courtesy of Kristina Micheva (Stanford)..
Calbindin (aka. calbindin D28k) is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is predominantly expressed in certain types of neurons, particularly in dendrites and perikarya of the cerebellum and is thought to buffer entry of calcium upon stimulation of glutamate receptors (Andressen et al., 1993). Calbindin has recently been shown to play a critical role in mitochondrial dysfunction and loss of synaptic proteins in vivo in an Alzheimer’s disease mouse model (Kook et al., 2014).
AT, ICC, IHC, WB
Fusion protein amino acids 1-261 (full-length) of human CALB1 (also known as Calbindin D28, Vitamin D-dependent calcium-binding protein, D-28K, Spot 35 protein, CAB27 and PCD-29, accession number P05937); Rat: 98% identity (257/261 amino acids identical); Mouse: 98% identity (257/261 amino acids identical); 60% identity with CALB2/Calretinin
Human, Mouse, Rat
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