Anti-ASIC1 Antibody (N271/44)

13 Citations

Our Anti-ASIC1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N271/44. It is KO validated, detects mouse and rat ASIC1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged ASIC1, ASIC2 or untagged Kv2.1 plasmid and probed with N271/44 (left) or N86/8 (right) TC supe.
Adult rat brain immunohistochemistry.
Immunoblot versus whole brain lysates from adult WT, ASIC1 KO and ASIC2 KO mice probed with N271/44 (left) and N52A/42 (right) TC supe. Mouse brain samples courtesy of Julie Saugstad (Robert S. Dow Neurobiology Laboratories, Legacy Research Institute).
Representative digitally generated blot of ASIC1 (cat. 75-277, 1:100) in dorsal root ganglion (DRG) tissue isolated from TRPV1+/+, TRPV1+/− and TRPV1−/− rats. For ASIC1 the bands shown at 64 and 89 kDa represent the non-glycosylated and glycosylated forms of ASIC1, respectively. There is no significant change in the expression of ASIC1 between the three groups. Image from publication CC-BY-4.0. PMID:37833914
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged ASIC1, ASIC2 or untagged Kv2.1 plasmid and probed with N271/44 (left) or N86/8 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged ASIC1, ASIC2 or untagged Kv2.1 plasmid and probed with N271/44 (left) or N86/8 (right) TC supe.
Adult rat brain immunohistochemistry.
Immunoblot versus whole brain lysates from adult WT, ASIC1 KO and ASIC2 KO mice probed with N271/44 (left) and N52A/42 (right) TC supe. Mouse brain samples courtesy of Julie Saugstad (Robert S. Dow Neurobiology Laboratories, Legacy Research Institute).
Representative digitally generated blot of ASIC1 (cat. 75-277, 1:100) in dorsal root ganglion (DRG) tissue isolated from TRPV1+/+, TRPV1+/− and TRPV1−/− rats. For ASIC1 the bands shown at 64 and 89 kDa represent the non-glycosylated and glycosylated forms of ASIC1, respectively. There is no significant change in the expression of ASIC1 between the three groups. Image from publication CC-BY-4.0. PMID:37833914

Human, Mouse, Rat
ICC, IHC, IP, WB
Mouse
490
550
594
650

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Data Sheet
KO Validated KO Validated

SKU: 75-277

Volume: 100 µL
1-2 business days
Price:
$319.00

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
PMID Dilution Publication
32458389not listedYang, J., et al. 2020. Acid-Sensing Ion Channels Contribute to Type III Adenylyl Cyclase-Independent Acid Sensing of Mouse Olfactory Sensory Neurons. Molecular Neurobiology, 3042-3056.
247880871:1000Vig, P.J., et al. 2014. Knockdown of acid-sensing ion channel 1a (ASIC1a) suppresses disease phenotype in SCA1 mouse model. Cerebellum, 479-190.
Immunoprecipitation: Human
PMID Dilution Publication
344617221.5ulLeisle, L., et al. 2021. Dynorphin Neuropeptides Decrease Apparent Proton Affinity of ASIC1a by Occluding the Acidic Pocket. Journal of Medicinal Chemistry, 13299-13311.
Western Blot: Human
PMID Dilution Publication
344617221:1500Leisle, L., et al. 2021. Dynorphin Neuropeptides Decrease Apparent Proton Affinity of ASIC1a by Occluding the Acidic Pocket. Journal of Medicinal Chemistry, 13299-13311.
340611611:1000Rook, M.L., et al. 2021. Mutation of a conserved glutamine residue does not abolish desensitization of acid-sensing ion channel 1. The Journal of General Physiology, .
339866471:1000Neuhof, A., et al. 2021. Large Acid-Evoked Currents, Mediated by ASIC1a, Accompany Differentiation in Human Dopaminergic Neurons. Frontiers in Cellular Neuroscience, 668008.
29057936not listedTian, Y., et al. 2017. Glioblastoma cancer stem cell lines express functional acid sensing ion channels ASIC1a and ASIC3. Scientific Reports, 13674.
27941930not listedChen, B., et al. 2016. ERK-mediated NF-κB activation through ASIC1 in response to acidosis.. Oncogenesis, e279.
Western Blot: Mouse
PMID Dilution Publication
290445281:100Farrag, M., et al. 2017. Endomorphins potentiate acid-sensing ion channel currents and enhance the lactic acid-mediated increase in arterial blood pressure: effects amplified in hindlimb ischaemia.. Journal of Physiology, 7167-7183.
Western Blot: Rat
PMID Dilution Publication
378783641:100Anselmi, L, et al. 2023. Functional knockout of the TRPV1 channel has no effect on the exercise pressor reflex in rats. The Journal of Physiology, .
313479221:30Kim, J.S., et al. 2019. Effect of knockout of the ASIC3 on cardiovascular reflexes arising from hindlimb muscle in decerebrated rats. American Journal of Physiology. Regulatory, Integrative, and Comparative Physiology, R641-R648.
264925271:200Diochot, S., et al. 2016. Analgesic effects of mambalgin peptide inhibitors of acid-sensing ion channels in inflammatory and neuropathic pain. Pain, 552-559.
230346521:300Diochot, S., et al. 2012. Black mamba venom peptides target acid-sensing ion channels to abolish pain.. Nature, 552-555.

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