Anti-Beta4-Spectrin Recombinant Chicken Chimeric mAb (N393/76)
Our chicken Anti-Beta4-spectrin recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N393/76. It detects human, mouse, and rat Beta4-spectrin and is purified by affinity chromatography. It works in WB, IHC, ICC, ELISA.
Representative section of formalin fixed, paraffin-embedded rat brain cerebral cortex showing cells stained by anti-Beta4 Spectrin recombinant chicken monoclonal antibody (green) (Cat No. 78-377). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-Beta4 Spectrin specifically stains the neuronal body and axon initial segment (AIS) in the cerebral cortex Magnification 100X.
Spectrin beta chain, non-erythrocytic 4, or ß4 spectrin, is a member of the spectrin family of cytoskeletal proteins. It is found in the cytoskeleton and cell cortex of cells in the brain and pancreatic islets. It is also expressed in skeletal muscule. ß4 spectrin functions as a scaffold protein that links the actin cytoskeleton to the plasma membrane. It is involved in cell shape and organization. Mutations in the ß4 spectrin gene are associated with congenital myopathy, and neurodevelopmental disorder with hypotonia, neuropathy and deafness (NEDHND).
Purified by affinity chromatography
1 mg/mL
Recombinant
N393/76
IgY
ELISA, ICC, IHC, WB
Chicken
SPTBN4 KIAA1642 SPTBN3
>200 kDa
Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin (accession number Q9H254) produced recombinantly in E. Coli
Human, Mouse, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N393/76 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N393/76 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
1X PBS, 0.05% Sodium Azide pH 7.4
WB: 1:1000
IHC: 1:250
ICC: 1:500
Unconjugated
Does not cross-react with other Beta-spectrins
Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
