Anti-Ankyrin-G (Staining) Recombinant Chicken Chimeric mAb (N106/36)
Our chicken Anti-Ankyrin-G (Staining) recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N106/36. It detects feline, hamster, human, mouse, non-human primate, and rat Ankyrin-G (Staining) and is purified by affinity chromatography. It works in WB, IHC, ICC, IP, ELISA.


Representative section of formalin fixed, paraffin-embedded rat brain cerebral cortex showing neurons stained by Ankyrin-G (ANK3) recombinant chicken monoclonal antibody (visualized in green) (Cat No. 78-146). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-Ankyrin-G specifically stains the neuronal cell body and axon initial segment (AIS) in the cerebral cortex. Mag. 100X.
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Feline, Hamster, Human, Mouse, Non-Human Primate, Rat
ELISA, ICC, IHC, IP, WB
Chicken

SKU: 78-146
Ships: 1-2 business days
Product Details
Ankyrin-G (Staining)
Ankyrin-G (ankyrin 3) is a member of the Ankyrin family which consists of Ankyrin R (1), Ankyrin B (2) and Ankyrin G (3) all of which are expressed in the nervous system. Ankyrins are structural proteins that aid in the attachment of integral membrane proteins with the spectrin-actin cytoskeleton, and thus targets voltage-gated sodium channels, potassium channels, cell adhesion molecules and others at the nodes of Ranvier and axonal initial segments (AIS). Variants in Ankyrin G have been linked to bipolar disorder.
Purified by affinity chromatography
1 mg/mL
Recombinant
N106/36
IgY
ELISA, ICC, IHC, IP, WB
Chicken
ANK3
>200 kDa
Fusion protein ~1000 amino acids of Ankyrin-G (accession number E9PE32) produced recombinantly in E. Coli
Feline, Hamster, Human, Mouse, Non-Human Primate, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N106/36 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N106/36 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
1X PBS, 0.05% Sodium Azide pH 7.4
WB: 1:1000
IHC: 1:500
ICC: 1:500
IHC: 1:500
ICC: 1:500
Unconjugated
Does not cross-react with Ankyrin-B.
Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Ankyrin-3 (ANK-3) (Ankyrin-G)