Anti-Tyrosine Hydroxylase (LNC1) Recombinant Chicken Chimeric mAb (LNC1)

Name: Anti-Tyrosine Hydroxylase (LNC1) Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-700
Price: 470.0 USD
Availability: InStock

Our chicken Anti-Tyrosine Hydroxylase (TH) recombinant monoclonal antibody is a chimeric antibody derived from clone LNC1. It detects chicken, frog, human, lizard, monkey, mouse, rat, vole, and zebrafish. Tyrosine Hydroxylase (TH) and is purified by affinity chromatography. It works in WB, IHC, ICC, IP, ELISA.

Representative section of formalin fixed, paraffin-embedded rat brain mid-brain region showing cells stained by anti-tyrosine hydroxylase (TH, clone LNC1) recombinant chicken monoclonal antibody (visualized in green, Cat No. 78-700). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-TH specifically stains the dopamine neurons in the mid-brain. Magnification 100X.

Western blotting of rat brain homogenate with Antibodies Incorporated anti-Tyrosine Hydroxylase mouse mAb (Cat No. 77-700) and Aves Labs anti-Tyrosine Hydroxylase recombinant chicken mAb (Cat No. 78-700) antibodies. The mouse mAb and chicken Mab were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. Aves Labs Recombinant Chicken anti-TH mAb recognizes endogenous Tyrosine Hydroxylase with similar sensitivity as the anti-TH mouse mAb in the rat brain homogenate at ~59-63 kDa.

Representative section of formalin fixed, paraffin-embedded rat brain mid-brain region showing cells stained by anti-tyrosine hydroxylase (TH, clone LNC1) recombinant chicken monoclonal antibody (visualized in green, Cat No. 78-700). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-TH specifically stains the dopamine neurons in the mid-brain. Magnification 100X.

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Species Reactivity

Chicken, Frog, Human, Lizard, Mouse, Non-Human Primate, Rat, Vole, Zebrafish

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

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Data Sheet

Trial Size

SKU: 78-700

Volume: 100 µL

Variant

Price:

$470.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Tyrosine Hydroxylase (TH)

Scientific Background

TH is the rate-limiting enzyme in the synthesis of the catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine and is responsible for converting L-tyrosine to L-dopa. Synthesis of catecholamines is regulated by the interaction of TH with its’ cofactor, tetrahydrobiopterin (BH4) and the substrates L-tyrosine and molecular oxygen. In humans four TH mRNA splice variants (hTH1-hTH4) have been isolated while subprimate species rely on a single form of TH. It is known that the hTH1-hTH4 variants are identical in their catalytic domain but differ in their N-terminal regulatory domains. Importantly, LNC1 reacts with the catalytic domain of TH and thus with all four isoforms of human TH. The role of TH in the synthesis of catecholamine neurotransmitters suggests a connection between the enzyme and a number of neuropathogenic diseases characterized by irregular catecholamine levels, such as Parkinson's disease, schizophrenia, and dystonia, as well as a variety of cardiovascular diseases.

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

LNC1

Isotype

IgY

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Gene Name

TH TYH EC 1.14.16.2

Molecular Weight

59-63 kDa

Antigen

Tyrosine Hydroxylase purified from PC12 cells

Species Reactivity

Chicken, Frog, Human, Lizard, Mouse, Non-Human Primate, Rat, Vole, Zebrafish

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone LNC1 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone LNC1 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Conjugate

Unconjugated

Specificity

Recognizes an epitope outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. Does not react with the following on Western Blots: purified dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT). Identifies a single ~60 kDa band on Western Blots of HeLa cells transfected to express human tyrosine hydroxylase.

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe rat brain lysate.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Tyrosine 3-monooxygenase

UniProt ID

P07101

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Anti-Tyrosine Hydroxylase (LNC1) Recombinant Chicken Chimeric mAb (LNC1)

SKU: 78-700

Product Details

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