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Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (Cat. p112-269, red, 1:1000). Nuclei labeled with DAPI. Magnification 10x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (Cat. p112-269, red, 1:1000) and total AQP2 (green). Nuclei labeled with DAPI. Magnification 600x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.
PhosphoSolutions Anti-Aquaporin 2 (Ser269) Antibody
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Western blot of Drosophila lysate showing specific labeling of the ~68 kDa AKT protein phosphorylated at Thr342 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-AKT (Thr342) Antibody
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Immunofluorescence of a section of mouse hippocampus colabeled with Anti-GFAP (Cat. 621-GFAP, green, 1:5000) and Anti-FOX3 (red). The Anti-FOX3 labels the nuclei and proximal perikarya of neurons while the Anti-GFAP labels a network of astroglial cells. The blue is DAPI staining of nuclear DNA.Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa GFAP protein.
PhosphoSolutions Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody
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Immunostaining of free-floating sections of mouse locus ceruleus (brainstem) labeling tyrosine hydroxylase (Cat. 2027-THSHP, red, 1:2000) The mouse was transcardially perfused with 20 mL of PBS containing 0.1% procaine and 100 U/mL heparin followed by 20 mL of 4% paraformaldehyde (PFA) in PBS. The dissected brains were kept in fixative at 4 °C overnight. The image was kindly provided by Eugene Dimitrov, Department of Physiology and Biophysics, Rosalind Franklin University.Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.
PhosphoSolutions Anti-Tyrosine Hydroxylase Antibody
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Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit polyclonal at 1:1000.Bar graph showing anti-Phosphoserine/threonine (PP2551) binding to a variety of phosphoserine and phosphothreonine peptides, but not control peptide containing unphosphorylated serine or phosphotyrosine.
PhosphoSolutions Anti-Phosphoserine/threonine Antibody
$340
Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (Cat. 322-COLT, 1:100, red) that has formed fibrils in the extracellular matrix. The blue is staining nuclear DNA.Immunofluorescence of a transversal section from mouse spinal cord labeling fibers of collagen (Cat. : 322-COLT, red, 1:200) from the tissue scaffold. The blue is DAPI counterstaining nuclear DNA. Image kindly provided by Dr. Rodolfo Gatto and Dr. Gerardo Morfini, Department of Anatomy and Cell biology, University of Illinois at Chicago.
PhosphoSolutions Anti-Collagen 1, alpha 1 telopeptide Antibody
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Western blot of rat olfactory bulb lysate showing specific immunolabeling of the ~53 kDa VGAT protein.
PhosphoSolutions Anti-Vesicular GABA transporter (VGAT) Antibody
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Western blot image of mouse gastrocnemius (lanes 1 & 3) and mouse diaphragm tissue lysate (lanes 2 & 4). The blot was probed with anti-Atrogin-1 (AP2041; lanes 1-4) in the presence (lanes 3 & 4) or absence (lanes 1 & 2) of Atrogin-1 peptide (AX2045).Formalin fixed, citric acid treated paraffin sections of E16 mouse skeletal muscle. Sections were probed with anti-Atrogin-1 (AP2041) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
PhosphoSolutions Anti-Atrogin-1 Antibody
$340
Western blot of 20 ug of mouse heart lysate showing specific immunolabeling of the ~25 kDa cardiac troponin I protein.Western blots showing specific labeling of S23/S24 TnI, cat. p2010-2324 (upper) and TnI; cat. 2010-TnI (lower) in protein lysates of mouse cardiomyocytes. Image from publication CC-BY-4.0. PMID: 36424916
PhosphoSolutions Anti-Troponin I (cardiac) Antibody
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Immunolabeling of a section of mouse piriform cortex labeled with Anti-Phospho-Ser133 CREB (Cat. p1010-133, red, 1:1000). Cell nuclei are visualized with DAPI DNA stain (blue).Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).
PhosphoSolutions Anti-CREB (Ser133) Antibody
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Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~78 kDa synapsin I doublet protein.Immunostaining of 40DIV cultured rat cortical neurons showing punctate labeling of synapsin (Cat. 1926-SYNP, 1:1000, red). The blue is staining nuclear DNA. Cells and photo courtesy of QBMCellScience.
PhosphoSolutions Anti-Synapsin I (100 µL) Antibody
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Western blot of human T47D cell lysate showing specific immunolabeling of the ~100 kDa CtIP phosphorylated at Thr847 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
PhosphoSolutions Anti-CtIP (Thr847) Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX3 protein.Immunofluorescence of a section of rat brain stem co-labeled with Anti-FOX3 (Cat. 583-FOX3, green, 1:1000) and Anti-MAP2 (Cat. 1100-MAP2, red, 1:5000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.
PhosphoSolutions Anti-FOX3 (NeuN) Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~48 kDa, ~65 kDa & ~75 kDa tau isoforms.Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-TAU antibody (Cat. 1998-TAU, green, 1:2000) and anti-MAP2 (red). The blue is DAPI staining nuclear DNA. Anti-TAU labels perikarya, dendrites, axons of neurons while anti-MAP2 only labels dendrites and perikarya of neurons. Where they overlap they appear orange-yellow.
PhosphoSolutions Anti-Tau Antibody
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Western blot of human striatal lysate showing specific immunolabeling of the ~88 kDa DAT protein.
PhosphoSolutions Anti-Dopamine Transporter, C-Terminus Antibody
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Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDA receptor.Immunostaining of rabbit retina showing NR2A in the rod and cone photoreceptors in the outer plexiform layer as well as the entire inner plexiform layer.
PhosphoSolutions Anti-NMDA NR2A Subunit (100 ul) Antibody
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Western blot analysis of mouse heart tissue (lanes 1 & 3) or C2C12 cells (lanes 2 & 4). The blot was probed with anti-MuRF1 (C-terminal region) (lanes 1 & 2) or anti-Atrogin-1 (lanes 3 & 4).Immunocytochemical labeling of MuRF1 in mouse C2C12 cells. The cells were labeled with rabbit polyclonal MuRF1 antibody, then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (MX3405).
PhosphoSolutions Anti-MuRF1 (C-terminal region) Antibody
$340
Western blot of HeLa cell lysates that had been treated with UV (~254 nm) for 0’ or 30’ showing the specific immunolabeling of the ~39 kDa p38 MAPK protein phosphorylated at Thr180/Tyr182.Immunostaining of human breast cancer tissue showing p38 when phosphorylated at Thr180/Tyr182 (red, 1:250). Photo courtesy of Patsy Ruegg.
PhosphoSolutions Anti-p38 MAPK (Thr180/Tyr182) Antibody
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Western blot of wild type (WT) beclin-1 expressed in HeLa cell lysate showing specific immunolabeling of beclin-1 phosphorylated at Ser234. This immunolabeling is decreased in the beclin-1 double alanine substitution mutant (S234A, S295A: Mut) expressed in HeLa cell lysate.
PhosphoSolutions Anti-Beclin1 (Ser234) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~105 kDa GluR1 protein.
PhosphoSolutions Anti-GluR1 Antibody
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Western blot of colchicine treated Sy5y cell lysate showing specific immunolabeling of the ~32 kDa cleaved actin fragment (fractin). Fractin (Cat. 592-FRAC, 1:5000; red) immunostaining of mouse brain signifying the emergence of damaged/dying neurons in entorhinal cortex (EC). Nuclei stained with DAPI (blue). Final image shows fractin+ cells (red) among YFP+ cells (green) at 7 dpi. Image from publication CC-BY-4.0. PMID:36468693
PhosphoSolutions Anti-Fractin Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68/70 kDa ChAT.
PhosphoSolutions Anti-ChAT (Choline Acetyltransferase) Antibody
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Immunostaining of mouse dentate gyrus showing beta III tubulin (red, 1:1000). The blue is staining nuclei. Photo courtesy of Robert Wine. Western blot of rat cortical lysate showing specific immunolabeling of the ~55 kDa beta III tubulin protein.
PhosphoSolutions Anti-Tubulin, beta III Antibody
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Western blot of 10ug of rat hippocampal lysate showing specific immunolabeling of the ~78 kDa synapsin I doublet protein.Immunostaining of cultured mouse caudate neurons showing punctate distribution of synapsin (Cat. 1925-SYNP, 1:1000, green) and MAP (red). Cells and photo courtesy of QBMCellScience.
PhosphoSolutions Anti-Synapsin I Antibody
$445

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