Anti-ERK1/2 (MAPK) (Thr-202/Tyr-204) [conserved], Phosphospecific Antibody (M206)

Name: Anti-ERK1/2 (MAPK) (Thr-202/Tyr-204) [conserved], Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: EM2061
Price: 370.0 USD
Availability: InStock

2 Citations

Our ERK1/2 (Thr-202/Tyr-204) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat ERK1/2 (Thr-202/Tyr-204) and is Protein A purified. It is great for use in WB, ICC.

Western blot analysis of human A431 epithelial cells untreated (lanes 1 & 4) or treated with 100 nM calyculin A for 30 min. (lanes 2 & 5) or 100 ng/ml EGF for 60 min. (lanes 3 & 6). The blots were probed with anti-ERK1 (C-terminal region) (lanes 1, 2, & 3) or anti-ERK1/2 (Thr-202/Tyr-204) (lanes 4, 5, & 6).

Immunocytochemical labeling of phosphorylated ERK1 in paraformaldehyde-fixed and NP-40-permeabilized rat A7r5 cells treated with calyculin A. The fixed cells were labeled with mouse monoclonal antibodies to anti-ERK1 (EM2331) and anti-ERK1/2 (Thr-202/Tyr-204) (EM2061). The antibodies were detected using Goat anti-Mouse secondary antibodies conjugated to DyLight® 488 (left) and DyLight® 594 (right).

Western blot analysis of human A431 epithelial cells untreated (lanes 1 & 4) or treated with 100 nM calyculin A for 30 min. (lanes 2 & 5) or 100 ng/ml EGF for 60 min. (lanes 3 & 6). The blots were probed with anti-ERK1 (C-terminal region) (lanes 1, 2, & 3) or anti-ERK1/2 (Thr-202/Tyr-204) (lanes 4, 5, & 6).

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

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Data Sheet

SKU: EM2061

Volume: 100 µL

Variant

Price:

$370.00

Quantity:

Ships: 1-2 business days

Product Details

Target

ERK1 (Thr-202/Tyr-204), ERK2 (Thr-185 and Tyr-187)

Scientific Background

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The ERK1/2 (p44/42) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK), a MAP kinase kinase (MAPKK), and a MAP kinase (MAPK). Multiple ERK1/2 MAPKKKs have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate ERK1 and ERK2 through phosphorylation of activation loop residues Thr-202/Tyr-204 and Thr-185/Tyr-187, respectively. ERK1/2 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases. Several downstream targets of ERK1/2 have been identified, including p90RSK and the transcription factor Elk-1.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M206

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

MAPK1

Molecular Weight

42/44

Immunogen

Clone M206 was generated from a dual phosphorylated ERK1 (Thr-202/Tyr-204) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Thr-202 and Tyr-204 in human ERK1. This sequence is conserved in rat and mouse ERK1, and is highly conserved in ERK2.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects 42 and 44 kDa* proteins corresponding to ERK1 and ERK2 on SDS-PAGE immunoblots of human A431 epithelial cells stimulated with EGF or calyculin A. It does not detect bands in control cells.

Post Translational Modification Type

GOmAb/Phosphorylated

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

ERK, p42, p44, MAPK

UniProt ID

P28482

Shipping

Blue Ice

Product Specific References for Applications and Species

Western Blot: Human | Mouse

Western Blot: Human
PMID	Dilution	Publication
23230267	not listed	Park, K., et al. 2013. A novel role of a lipid species, sphingosine-1-phosphate, in epithelial innate immunity. Molecular and Cellular Biology, 752-762.

Western Blot: Mouse
PMID	Dilution	Publication
30800127	not listed	Elizondo, D, et al. 2019. Allograft Inflammatory Factor-1 Governs Hematopoietic Stem Cell Differentiation Into cDC1 and Monocyte-Derived Dendritic Cells Through IRF8 and RelB in vitro. Frontiers in immunology, 173.

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Anti-ERK1/2 (MAPK) (Thr-202/Tyr-204) [conserved], Phosphospecific Antibody (M206)

SKU: EM2061

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-ERK1/2 (MAPK) (Thr-202/Tyr-204) [conserved], Phosphospecific Antibody

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