Anti-Ephexin-1 (C-terminal region) Antibody

Name: Anti-Ephexin-1 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: EP2821
Price: 340.0 USD
Availability: InStock

4 Citations

Our Ephexin-1 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Ephexin-1 (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot analysis of human A431 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-Ephexin-1 (C-terminal region) (lanes 1 & 2) and anti-Ephexin-1 (Tyr-87) (lanes 3 & 4).

Immunocytochemical labeling of phosphorylated Exphexin-1 in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal Ephexin-1 (C-terminal region) and Ephexin-1 (Tyr-87) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Western blot analysis of human A431 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-Ephexin-1 (C-terminal region) (lanes 1 & 2) and anti-Ephexin-1 (Tyr-87) (lanes 3 & 4).

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Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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SKU: EP2821

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Ephexin-1 (C-terminal region)

Scientific Background

The Eph family of receptor tyrosine kinases, and their ephrin ligands, are important for cell positioning and morphogenesis during development. EphA4 receptor can inhibit axon outgrowth and has roles in regulating axon projections during neural development. Eph signaling pathways require both receptor kinase activity and activation of Rho-GTPase guanine nucleotide-exchange factors (GEFs). Ephexin-1 is a Dbl family GEF that may be important for regulating Rho GTPase activity downstream of EphA4/FGFR complexes. EphA4 activation leads to Src kinase phosphorylation of Tyr-87 in ephexin-1, which enhances activation of RhoA, but not Rac1 or Cdc42. In addition, ephexin-1 also binds FGFRs, and is phosphorylated at Tyr-87, as well as other tyrosine sites.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

NGEF

Molecular Weight

Immunogen

Ephexin-1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of mouse ephexin-1. This sequence has significant homology to the same region in human and rat ephexin-1, and has low homology to other known proteins.

Immunogen Species

Mouse

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:50

Conjugate

Unconjugated

Specificity

The antibody detects an 80 kDa* band corresponding to ephexin-1 in Western blots of human A431, Jurkat, and HUVEC cells, as well as rat PC12 cells and mouse brain tissue.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

EphA, Eph, NGEF

UniProt ID

Q8CHT1

Shipping

Blue Ice

Product Specific References for Applications and Species

Immunocytochemistry: Chicken
Immunohistochemistry: Rat
Western Blot: Chicken | Mouse | Rat

Immunocytochemistry: Chicken
PMID	Dilution	Publication
30237102	not listed	Fiore, L., et al. 2019. Regulation of axonal EphA4 forward signaling is involved in the effect of EphA3 on chicken retinal ganglion cell axon growth during retinotectal mapping. Experimental Eye Research, 46-60.

Immunohistochemistry: Rat
PMID	Dilution	Publication
20949525	1:500	Rosas, O.R., et al. 2011. Expression and activation of ephexin is altered after spinal cord injury. Developmental Neurobiology, 595-607.

Western Blot: Chicken
PMID	Dilution	Publication
30237102	not listed	Fiore, L., et al. 2019. Regulation of axonal EphA4 forward signaling is involved in the effect of EphA3 on chicken retinal ganglion cell axon growth during retinotectal mapping. Experimental Eye Research, 46-60.

Western Blot: Mouse
PMID	Dilution	Publication
21339325	not listed	Muto, A., et al. 2011. Eph-B4 prevents venous adaptive remodeling in the adult arterial environment. Journal of Experimental Medicine, 561-575.

Western Blot: Rat
PMID	Dilution	Publication
25657738	1:1000	Rosas, O.R., et al. 2014. Long-term treatment with PP2 after spinal cord injury resulted in functional locomotor recovery and increased spared tissue. Neural Regen Research, 2164-2173.
20949525	1:1000	Rosas, O.R., et al. 2011. Expression and activation of ephexin is altered after spinal cord injury. Developmental Neurobiology, 595-607.

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Anti-Ephexin-1 (C-terminal region) Antibody

SKU: EP2821

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Ephexin-1 (C-terminal region) Antibody

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