Cell Technology

29 products

In 2022, Cell Technology merged with ImmunoChemistry Technologies and Antibodies Inc to advance its mission of developing unique, cell-permeable assays for studying cellular functions.

Founded in 1998 and specializing in in situ detection of tissue-specific events while preserving cell morphology and architecture, Cell Technology’s trusted products, including its first line detecting apoptotic cells via active caspases and mitochondrial membrane potential loss, have empowered researchers in academia, biotechnology, and pharmaceuticals.

This merger enhances our capacity to deliver innovative reagents. Cell Technology's products remain unchanged in quality and performance, ensuring researchers can rely on consistent results. We are dedicated to enhancing your research experience, and their extensive range of dependable products perfectly complements ImmunoChemistry and Antibodies Inc's robust portfolio of cell viability assays and services.

Showing 1 - 24 of 29 products

Figure 1. Assay principle. The kit uses a non-fluorescent detection reagent, which is reduced by NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction (which does not react with NADP/NADPH).

Figure 2. NADH standard curve. Titrated in NADH standard diluent. Incubation time was 1 hour.

ImmunoChemistry Technologies Fluoro NAD/NADH Detection Kit by Cell Technology

From $609

Figure 1. (a) Fluorescent responses of NP3 (5 μM) toward various analytes (10 μM). Data shown represent fluorescent intensity at 470nm, 30 min after addition of the analytes. (b) ONOO− (final 10 μM) was quickly injected into a solution of NP3 (final 5μM), and the fluorescent intensity at 470 nm was plotted against time. (c) Fluorescence enhancement of NP3 (5μM) at 470 nm as a function of ONOO− (0−10 μM) after 15 min of reaction. All data acquired in PBS (10 mM, pH 7.4) with excitation at 375 nm.

Figure 2. (a) Time-lapse images taken from living EA.hy926 endothelial cells. Cells were preincubated with NP3 (5.0 μM), followed by stimulation with or without SIN-1 (0.5 mM). (b) Dynamic changes of NP3 fluorescence after SIN-1 (0.5 mM) treatment in panel a. (c) Effects of ONOO− scavengers uric acid (100 μM) and FeTTPS (1 μM) on changes in NP3 fluorescence in endothelial cells in the presence of ONOO− (60 μM). PI (red) stains nuclei. NP3 fluorescence was collected at 420−480 nm with λex 405 nm.

ImmunoChemistry Technologies Peroxynitrite Detection Kit

$298

Figure 1. Assay principle. The kit uses a non-fluorescent detection reagent to measure H2O2. H2O2 oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin.

Figure 2. Typical Hydrogen peroxide standard curve. A new curve must be generated each time the assay is run.

ImmunoChemistry Technologies Hydrogen Peroxide Detection/Peroxidase Detection Kit

$288

5000 Ramos cells/well were incubated with serially diluted Rituxan antibody for 15 minutes prior to the addition of purified NK cells stimulated overnight with IL-2. The ADCC reaction was further incubated for 2 hours at the specified E:T ratios. % Cytotoxicity was measured using the aCellaTOX assay.

ImmunoChemistry Technologies aCella - Tox - an assay designed to measure cell cytoxicity

From $910

Figure shows the two phosphate moieties of ADP, with no trace of a peak indicating the third phosphate of ATP using P31 NMR.

ImmunoChemistry Technologies Ultra Pure ADP by Cell Technology

From $405

Figure 1. Assay principle: a non‐fluorescent detection reagent is oxidized in the presence of hydrogen peroxide and MPO to produce its fluorescent analog.

Figure 2. A MPO standard curve was prepared and run, as described in the protocol, in the absence (A) or presence (B) of 20 mM catalase inhibitor. MPO standard and reaction mix were added to a 96-well black plate and incubated at room temperature in the dark for 30 minutes. The wells were read using Ex: 530nm and Em: 590nm. There is an approximately 50% reduction in signal in the presence of 20mM catalase inhibitor (note the different scales on the x-axis).

ImmunoChemistry Technologies Myeloperoxidase Detection Kit

$522

Figure 2. Typical standard curve. A new curve must be generated each time the assay is run.

ImmunoChemistry Technologies Fluorescent Sarcosine Detection Kit

$368

Figure 2. Bovine serum Semicarbazide-sensitive amine oxidase was serially diluted in 1X Reaction buffer. The serially diluted samples were run as described in the protocol. The samples were read after a 3 hours incubation period. Excitation: 530 nm and emission: 590 nm.

ImmunoChemistry Technologies Fluorescent Semicarbazide-Sensitive Amine Oxidase Detection Kit

$490

Figure 2. Phosphate standard curve was generated using fluorometric detection: excitation 535 nm and emission 585 nm. Incubation time = 60 minutes at 37°C. Standard curve range 1.5625 μM to 100μM. Graph was plotted using 4PL non-linear regression.

ImmunoChemistry Technologies Fluorescent Phosphate Detection Kit

$278

ImmunoChemistry Technologies Fluorescent Monoamine Oxidase Detection Kit

$298

ImmunoChemistry Technologies Fluorescent Lactate Detection Kit

$442

Figure 1. Staurosporine induced Jurkat cell viability assay. Titrated doses of staurosporine were added into seeded Jurkat cells (10,000 cells/well) in 96-well black opaque tissue culture plates and incubated at 37°C, 10% CO2 for 24h. Dye reagent (1/10 volume) was added into the cultured cells and incubated for 3h and fluorescence was detected at Ex: 530 nm and Em: 590 nm.

Figure 2. PBMC proliferation in response to mitogen Concanavalin A (Con-A) stimulation measured using Fluorescent Cell Proliferation Assay. PBMC at 15,000 cells were cultured for 3 days in the presence of titrated amounts of Con A and tested for proliferation using the Fluorescent Cell Proliferation Assay. Dye reagent (1/10 volume) was added to the cultured cells and incubated at 37°C for 2h. Fluorescence was detected at Ex: 530 nm and Em: 590 nm.

ImmunoChemistry Technologies Fluorescent Cell Proliferation Assay

From $118

Figure 2. The reaction contained 20mM hydrogen peroxide (final) per well and the indicated amounts of catalase. The reaction was incubated for 30 minutes at room temperature. Reaction cocktail was added to each well and the reaction incubated for another 10 minutes in the dark at room temperature. Fluorescence was measured at excitation 530nm and emission 590nm. The graphs show the net fluorescence change: Negative Control fluorescence (no catalase) minus Sample (Cat.alase) fluorescence.

ImmunoChemistry Technologies Fluoro Catalase Activity Detection Kit by Cell Technology

$352

ImmunoChemistry Technologies Fluorescent ATP Detection Kit

From $405

ImmunoChemistry Technologies Fluorescent ADP Detection Kit

From $405

ImmunoChemistry Technologies Fluoro AChE - a fluorescence assay for monitoring and detecting acetylcholinesterase activity in samples

From $501

Figure 1. Standard curve (fluorescence). Excitation 535 nm and emission 585 nm. R2 value=0.9977. Incubation time= 1 hour at room temperature. Standard curve range 0.15625 μM to 10μM. A new curve must be generated each time the assay is run.

Figure 2. Standard curve (absorbance). Absorbance read-out at 570 nm. R2 =0.9993. Incubation time = 30 minutes. Standard curve range 1.25μM to 80μM. A new curve must be generated each time the assay is run.

ImmunoChemistry Technologies Fluorescent Total Cholesterol Detection Kit

$368

Figure 2. Comparison of NADPH & NADH Standard Curves

ImmunoChemistry Technologies Fluorescent NADP/NADPH Detection Kit

From $609

ImmunoChemistry Technologies Chlorination and Oxidation Detection Kit by Cell Technology

$703

Figure 1. Jurkat cells were stimulated with Staurosporine for 3 hours (B) or DMSO (A). Mito Flow reagent was added according to the protocol, incubated for 30 minutes and analyzed by flow cytometry: Ex:488nm Em: FL2 channel.

ImmunoChemistry Technologies Mito Flow - Mitochondrial membrane potential detection kit

From $288

Figure 2. Detection of Hypochlorite ( -OCl ) in neutrophils. Neutrophils were isolated from porcine blood, washed in Krebs-Ringers phosphate buffer and seeded in glass bottom dishes. The cells were then loaded with APF (10μM final) by incubation for 30 minutes at room temperature. The Dye-loaded neutrophils were stimulated with PMA (2ng/mL). Fluorescence images were acquired before and 10 minutes after stimulation. Excitation: 488nm emission: 505-550 nm barrier filters

ImmunoChemistry Technologies Highly Reactive Oxygen Species (hROS) Detection Kit

$298

Figure 1. Detection of Hypochlorite ( -OCl ) in neutrophils. Neutrophils were isolated from porcine blood, washed in Krebs-Ringers phosphate buffer (as described in step V 1. above) and seeded in glass bottom dishes. The cells were then loaded with APF or HPF (10 mM final) by incubation for 30 minutes at room temperature. The Dye-loaded neutrophils were stimulated with PMA (2ng/mL).

Figure 2. Fluorescence images were acquired before and 10 minutes after stimulation. Excitation: 488 nm emission: 505-550 nm barrier filters. Hypochlorite production can be detected with increase APF fluorescence and no increase in HPF fluorescence.

ImmunoChemistry Technologies Fluorescent Hypochlorite Detection Kit

$522

Figure 1. Jurkat cells were stimulated with staurosporine (B) or DMSO (A), then stained according to the kit protocol. The cells were washed twice and analyzed by flow cytometry: Ex:488nm Em: FL1 and FL2. A. Healthy cells show a strong red fluorescence (intact mitochondria) and no green fluorescence (no active caspases). B. Apoptotic cells show a loss of red fluorescence (y axis) due to loss of mitochondrial membrane potential and positive green fluorescence (x axis) indicating active caspases.

Figure 2. HeLa Cells activated with staurosporine (B) or DMSO (A), then stained according to the kit protocol. The cells were washed twice and analyzed by flow cytometry: Ex:488nm Em: FL1 and FL2. A. Healthy cells show a strong red fluorescence (intact mitochondria) and no green fluorescence (no active caspases). B. Cells becoming apoptotic show loss of red fluorescence (y axis) due to loss of mitochondrial membrane potential and positive green fluorescence (x axis) indicating active caspases.

ImmunoChemistry Technologies Dual Caspase 1 and Mitochondrial Membrane Potential Assay Kit

From $195

ImmunoChemistry Technologies Dual Caspase 3/7 and Mitochondrial Membrane Potential Assay Kit

From $195

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