Anti-Growth Associated Protein 43 (GAP-43) Antibody (GAP43)

Our Anti-Growth associated protein 43 (GAP-43) mouse monoclonal primary antibody detects mouse and rat Growth associated protein 43 (GAP-43), and is IgG. It is validated for use in FC, ICC, WB.

A: Flow Cytometry analysis of GAP43 expressed in human neuroblastoma SH-SY5Y cell line. Fixing and permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS; Blocking: 1% BSA; Primary antibody: Mouse Monoclonal antibody to GAP43 (cat # M-1650-100, 2 μg per ~106 cells) for 30 minutes at room temperature; Secondary antibody: Goat anti-mouse PE (1:100 dilution), incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-200) was used as negative control under same conditions. Data and results were generated using Orflo MoxiflowTM instrument and protocols. B: Immunofluorescence analysis of mixed neuron-glial cultures stained with mouse anti-GAP43 (green) and rabbit anti-MAP2 (red). Blue: DNA staining. The GAP43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites. C: Western Blot analysis of GAP43 expression in whole rat spinal cord lysates. The antibody recognizes the ~43 kDa protein.
A: Flow Cytometry analysis of GAP43 expressed in human neuroblastoma SH-SY5Y cell line. Fixing and permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS; Blocking: 1% BSA; Primary antibody: Mouse Monoclonal antibody to GAP43 (cat # M-1650-100, 2 μg per ~106 cells) for 30 minutes at room temperature; Secondary antibody: Goat anti-mouse PE (1:100 dilution), incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-200) was used as negative control under same conditions. Data and results were generated using Orflo MoxiflowTM instrument and protocols. B: Immunofluorescence analysis of mixed neuron-glial cultures stained with mouse anti-GAP43 (green) and rabbit anti-MAP2 (red). Blue: DNA staining. The GAP43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites. C: Western Blot analysis of GAP43 expression in whole rat spinal cord lysates. The antibody recognizes the ~43 kDa protein.
A: Flow Cytometry analysis of GAP43 expressed in human neuroblastoma SH-SY5Y cell line. Fixing and permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS; Blocking: 1% BSA; Primary antibody: Mouse Monoclonal antibody to GAP43 (cat # M-1650-100, 2 μg per ~106 cells) for 30 minutes at room temperature; Secondary antibody: Goat anti-mouse PE (1:100 dilution), incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-200) was used as negative control under same conditions. Data and results were generated using Orflo MoxiflowTM instrument and protocols. B: Immunofluorescence analysis of mixed neuron-glial cultures stained with mouse anti-GAP43 (green) and rabbit anti-MAP2 (red). Blue: DNA staining. The GAP43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites. C: Western Blot analysis of GAP43 expression in whole rat spinal cord lysates. The antibody recognizes the ~43 kDa protein.
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Mouse, Rat
Flow, ICC, WB
Mouse

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Data Sheet

SKU: M-1650-100

Volume/Size: 100 µg
Price:
$327.00

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