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Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of NCAM1. The sections were stained with anti-NCAM1 chicken polyclonal antibody (visualized in green) at 1 µg/mL (1:200) dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat no. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Magnification 100X.Western blotting of rat brain homogenate with chicken anti-NCAM1 antibody and detected with goat anti-chicken HRP. Chicken anti-NCAM1 recognizes endogenous NCAM1 in rat brain homogenate at ~180 kDa.
Aves Labs Anti-NCAM1 Antibody
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Immunofluorescent staining of mock- or 50 µM chloroquine-treated HeLa cells using 2 µg/mL chicken anti-SQSTM1 (p62) antibody (green). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat No. AR-6501). Anti-SQSTM1 (p62) specifically stains the autophagosomes in HeLa cells. ​Chloroquine treatment increases the levels of SQSTM1 (p62) protein by inhibiting autophagy.Western blotting of mock- or 50 µM chloroquine-treated HeLa cell lysates with Aves Labs chicken anti-SQSTM1 (p62) antibody at 1:5000 (0.04 µg/ml) and 1:10000 (0.02µg/ml) dilutions and detected with goat anti-chicken HRP. Aves Labs Chicken anti-SQSTM1 (p62) recognizes endogenous SQSTM1 (p62) in HeLa cells at ~62 kDa. Chloroquine treatment increases the levels of SQSTM1 (p62) protein by inhibiting autophagy.
Aves Labs Anti-SQSTM1 Antibody
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Immunofluoresence of COS7 cells expressing mScarlet3 using chicken anti-mScarlet3 antibody (green) and showing mScarlet3 autofluorescence (red). The cells were mounted with ICT's Fluoroshield with DAPI mounting medium (Cat. AR-6501). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells. The staining revealed a complete overlap between the signal from chicken anti-mScarlet3 antibody and mScarlet3 autofluoresence in transfected cells.Western blotting of mock or mScarlet3 transfected COS-7 cell lysates with chicken anti-mScarlet3 antibody (0.5 ug/ml) and detected with anti-chicken HRP. Chicken anti-mScarlet3 recognizes exogenous mScarlet3 in COS-7 cells.​
Aves Labs Anti-mScarlet3 Antibody
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Immunofluorescent staining of MCF7 cells using 1 µg/mL chicken anti-HB9 (MNX1) antibody (green). Actin filaments were stained using Phalloidin (red). The cells were mounted with ICT's Fluoroshield mounting medium (Cat no. AR-6500). Anti-HB9/MNX1 specifically stains the nucleus of MCF7 cells.​Western blotting of MCF7 cell lysates with  chicken anti-HB9 (MNX1) antibody at various concentrations and detected with anti-chicken HRP. Chicken anti-HB9/MNX1 recognizes endogenous MNX1 in MCF7 cell lysates at ~50 kDa.
Aves Labs Anti-MNX1/HB9 Antibody
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COS-7 cells expressing NDRG2-FLAG were subjected to immunofluorescent staining using Aves Labs chicken anti-NDRG2 antibody (visualized in green) and rabbit anti-FLAG antibody (visualized in red). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells. The staining revealed a complete overlap between the signal from the chicken anti-NDRG2 antibody and the anti-FLAG antibody specifically in transfected cells.Western blotting of mock or NDRG2-FLAG transfected COS-7 cell lysates and human, mouse, and rat brain homogenates (10 ug/lane) with Aves Labs chicken anti-NDRG2 antibody (0.2 ug/ml) and detected with anti-chicken HRP. Anti-NDRG2 recognizes endogenous NDRG2 in brain homogenates in addition to exogenous NDRG2 in COS-7 cells.
Aves Labs Anti-NDRG2 Antibody
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Adult mouse DRG was fixed in 4% paraformaldehyde, cryostat-sectioned, and then stained for PAP immunoreactivity (1:500 dilution), showing immunoreactive material in primary sensory neurons. Photomicrograph by Dr. Mark Zilka, Univ. of North Carolina.Adult mouse spinal cord was fixed in 4% paraformaldehyde, paraffin-embedded and sectioned, and then sections were stained for PAP immunoreactivity (1:500 dilution). Adjacent sections were co-stained for IB4 and Calcitonin Gene-Related Protein (CGRP) (other sensory neuronal markers). Photomicrograph by Dr. Mark Zilka, University of North Carolina.
Co-staining of Coronin1 (COR, green, 1:1000 dilution) and Internexin (rabbit antibody, red, 1:500) in a culture of mouse cerebral cortical tissue fixed with 4% paraformaldehyde. Photo by Dr. Gerry Shaw, Univ. Florida.Co-staining of Coronin1 (COR, green, 1:1000 dilution) and Internexin (rabbit antibody, red, 1:500) in a culture of mouse cerebral cortical tissue fixed with 4% paraformaldehyde. Photo by Dr. Gerry Shaw, Univ. Florida.

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