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In September, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of September citations from 2020.

Magupalli VG, Negro R, Tian Y, Hauenstein AV, Di Caprio G, Skillern W, Deng Q, Orning P, Alam HB, Maliga Z, Sharif H, Hu JJ, Evavold CL, Kagan JC, Schmidt FI, Fitzgerald KA, Kirchhausen T, Li Y, Wu H. HDAC6 mediates an aggresome-like mechanism for NLRP3 and pyrin inflammasome activation. Science. 2020 Sep 18;369(6510):eaas8995. doi: 10.1126/science.aas8995. Abstract

"Membrane permeability was measured by PI exclusion assay. Untreated and treated cells were collected, including dead floating cells in the medium, and washed two times in PBS before resuspension in 2 mg/ml PI (ImmunoChemistry Technologies, catalog no. 638). The percentage of cells that took up PI was measured by flow cytometry (BD Biosciences, FACSAria II)."

Shirane M, Wada M, Morita K, Hayashi N, Kunimatsu R, Matsumoto Y, Matsuzaki F, Nakatsumi H, Ohta K, Tamura Y, Nakayama KI. Protrudin and PDZD8 contribute to neuronal integrity by promoting lipid extraction required for endosome maturation. Nat Commun. 2020 Sep 11;11(1):4576. doi: 10.1038/s41467-020-18413-9. Full Text

"The retroviruses in the resulting culture supernatants were used to infect HeLa cells, and the cells were then subjected to selection with puromycin (2 μg/ml). Magic red was obtained from Immunochemistry Technologies (Bloomington, MN)."

Broday L, Adir O, Bening-Abu-Shach U, Arbib S, Henis-Korenblit S. Inactivation of the Caenorhabditis elegans RNF-5 E3 ligase promotes IRE-1-independent ER functions. Autophagy. 2020 Sep 26. doi: 10.1080/15548627.2020.1827778. Online ahead of print. Abstract

"Magic Red (ImmunoChemistry Technologies, 6133) produces red fluorescence following proteolytic cleavage by cathepsin B in functional lysosomes."

Verma D, Fekri SZ, Sigurdardottir G, Eding CB, Sandin C, Enerbäck C. Enhanced inflammasome activity in patients with psoriasis promotes systemic inflammation. J Invest Dermatol. 2020 Sep 4;S0022-202X(20)31943-6. doi: 10.1016/j.jid.2020.07.012. Online ahead of print. Full Text

"Peripheral blood samples were left untreated or treated with LPS for 1 hour at 37°C. The RBCs were lysed, and stained with CD4-Pacific blue, CD14-APC-Cy7, CD8-PE and CD16- PE-Cy7 (all from BD Biosciences). Caspase-1 activity was determined using a caspase-1 FLICA (Fluorescence inhibitor of caspase-1) kit and caspase-3/7 activity was detected using FLICA 660 (ImmunoChemistry Technologies, Bloomington, MN, USA) according to the manufacturer’s protocol."

Elsesy ME, Oh-Hohenhorst SJ, Löser A, Oing C, Mutiara S, Köcher S, Meien S, Zielinski A, Burdak-Rothkamm S, Tilki D, Huland H, Schwarz R, Petersen C, Bokemeyer C, Rothkamm K, Mansour WY. Second-Generation Antiandrogen Therapy Radiosensitizes Prostate Cancer Regardless of Castration State through Inhibition of DNA Double Strand Break Repair. Cancers. 2020 Aug 31;12(9):E2467. doi: 10.3390/cancers12092467. Full Text

"Apoptosis was investigated by detection of caspase activity utilizing the FAMFLICA ™ Poly Caspases Assay Kit (Immunochemistry Technologies, Bloomington, MN, USA), according to the manufacturer’s instructions. Flow cytometric analysis was performed on a FACS Canto with FACS Diva Software (Becton Dickin-son, Toronto, ON, Canada). Staurosporine (Sigma S6942) was used as a positive control with a final concentration of 1 µM for at least 12 h incubation."

Maique J, Flores B, Shi M, Shepard S, Zhou Z, Yan S, Moe OW, Hu MC. High Phosphate Induces and Klotho Attenuates Kidney Epithelial Senescence and Fibrosis. Front Pharmacol. 2020 Aug 20;11:1273. doi: 10.3389/fphar.2020.01273. eCollection 2020. Full Text

"The culture media were harvested for lactate dehydrogenase (LDH) measurement with LDH Cytotoxicity Assay Kit (LDH cytotoxicity detection kit (Clontech Laboratories, Mountain View, CA) to evaluate cell injury, and for 8-hydroxydeoxyguanosine (8-OHdG) measurement with DNA Damage (8-OHdG) ELISA kit (ImmunoChemistry Technologies, Bloomington, Minnesota, USA) to evaluate oxidative DNA damage following the manufacturer’s protocols."

Products Used:
Propidium Iodide Stain
Magic Red Cathepsin K Assay
Magic Red Cathepsin B Assay
FAM-FLICA Caspase-1 Assay
FLICA 660 Caspase-3/7 Assay
FAM-FLICA Poly Caspase Assay
DNA Damage ELISA Kit