DNA Damage ELISA Kit

Assess DNA damage by measuring 8-hydroxy-2-deoxyguanosine in urine, cell culture, plasma and other sample matrices. This kit offers a quick incubation time, stable reagents, and a user-friendly protocol. Analyze using an absorbance plate reader.

Figure 1. Oxidation of Guanosine
Figure 2. Recovery of 8-OHdG from urine. Urine samples were spiked with 8-OHdG, diluted as described in the protocol, analyzed using the 8-OHdG ELISA Kit. The y-intercept corresponds to the amount of 8-OHdG in unspiked urine. Error bars represent standard deviations obtained from multiple dilutions of each sample.
Figure 3. Typical standard curve. A new standard curve must be generated for each assay.
Figure 4. Assay overview
Figure 1. Oxidation of Guanosine
Figure 1. Oxidation of Guanosine
Click on image to zoom

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Product Manual
Safety Data Sheet

SKU: 9143

Size: 1 96-Well Plate
Price:
$677.00
Ships: 1-2 business days
  1. Prepare the standard and samples in the Sample and Standard Diluent.
  2. Add 50 µL of prepared standards and samples in triplicate to appropriate wells.
  3. Add 50 µL of the diluted antibody preparation to the appropriate wells.
  4. Cover plate with Plate Cover and incubate at room temperature (20-25°C) for 1 hour.
  5. Dilute 10X Wash Buffer 1:10 with diH2O.
  6. Wash plate 4 times with 1X Wash Buffer.
  7. Add 100 µL TMB Substrate to each well.
  8. Cover plate with Plate Cover and incubate at room temperature in the dark for 30 minutes to develop the signal.
  9. Add 100 µL Stop Solution to each well.
  10. Measure absorbance using a plate reader at 450 nm.
  11. Plot the standard curve and calculate sample concentrations.
  • 8-OHdG: BSA Coated 96-Well Plate, #270
  • 2 Plate Covers, #271
  • 8-OHdG, 100 µL at 3.06 µg/mL, #6646
  • 8-OHdG HRP Conjugated Monoclonal Antibody, 75 µL, #6647
  • Sample and Standard Diluent, 50 mL, #6648
  • 8-OhdG Antibody Diluent, 13 mL, #6649
  • 10X Wash Buffer Concentrate, 50 mL, #6650
  • TMB Substrate, 13 mL, #6651
  • Stop Solution, 13 mL, #6652
  • Kit Manual

    • Bulk Order DNA Damage ELISA Kit

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