Products: Primary Antibodies

Anti-Flrt3 (N353/13)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-328	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-328	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse

Applications:

ICC

Immunogen:

Fusion protein amino acids 29-526 (extracellular domain) of mouse Flrt3 (also known as Leucinerich repeat transmembrane protein FLRT3, Fibronectin-like domain-containing leucine-rich transmembrane protein 3, KIAA1469 and UNQ856/PRO1865, accession number Q8BGT1)
Rat: 99% identity (496/498 amino acids identical)
Human: 96% identity (480/498 amino acids identical)
50% identity with Flrt1 and Flrt2

Cross Reactivity:

No cross-reactivity against Flrt1 or Flrt2

Protein Name(s):

Leucine-rich repeat transmembrane protein FLRT3 (Fibronectin leucine rich transmembrane protein 3)

Gene Name(s):

Flrt3 Kiaa1469

Antibody Registry ID:

Purified: AB_2315833
TC Supernatant: AB_2315832

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.