Chicken Annexin V-Fluorescein Apoptosis Assay Kit

For quickly and easily distinguishing two populations of dying cells from viable cells using recombinant fluorescein-conjugated Chicken Annexin V and Propidium Iodide. Results can be analyzed via flow cytometer.

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), washed, then stained with Chicken Annexin V-Fluorescein Assay (Cat. 9140). Chicken Annexin V-Fluorescein labeled only 16.9% of control cells (UR + LR = 13.2% + 3.7%, left); staurosporine induced Chicken Annexin V-Fluorescein labeling in 97% of cells (UR + LR = 46.6% + 50.4%, right). Staurosporine increased the proportion of dual stained cells (UR). Data courtesy of Mrs. Tracy Murphy, ICT, 227:19.
Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), washed, then stained with Chicken Annexin V-Fluorescein Assay (Cat. 9140). Chicken Annexin V-Fluorescein labeled only 16.9% of control cells (UR + LR = 13.2% + 3.7%, left); staurosporine induced Chicken Annexin V-Fluorescein labeling in 97% of cells (UR + LR = 46.6% + 50.4%, right). Staurosporine increased the proportion of dual stained cells (UR). Data courtesy of Mrs. Tracy Murphy, ICT, 227:19.
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Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), washed, then stained with Chicken Annexin V-Fluorescein Assay (Cat. 9140). Chicken Annexin V-Fluorescein labeled only 16.9% of control cells (UR + LR = 13.2% + 3.7%, left); staurosporine induced Chicken Annexin V-Fluorescein labeling in 97% of cells (UR + LR = 46.6% + 50.4%, right). Staurosporine increased the proportion of dual stained cells (UR). Data courtesy of Mrs. Tracy Murphy, ICT, 227:19.

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Product Manual
Safety Data Sheet

SKU: 9140

Size: 500 Tests
Price:
$469.00
Ships: 1-2 business days
  1. Prepare samples and controls.
  2. Dilute 10X Binding Buffer 1:10 WITH DiH2O.
  3. Wash cells twice in ice-cold culture medium or PBS and resuspend in ice-cold 1X Binding Buffer at 5 x 105 – 1 x 106 cells/mL.
  4. Reconstitute Chicken Annexin V-Fluorescein in 1 mL Annexin Reconstitution Buffer, 1X.
  5. Dilute reconstituted Annexin V-Fluorescein 1:5 in PBS.
  6. Add diluted Annexin V-Fluorescein to each sample at ~1:10.
  7. Dilute PI 1:10 in PBS.
  8. Add diluted PI to each sample at ~1:20.
  9. Incubate 15 minutes on ice and protected from light.
  10. Add 400 µL 1X Binding Buffer to each sample.
  11. Analyze with a flow cytometer. Annexin V-Fluorescein excites at 494 nm and emits at 521 nm. PI excites at 536 nm and emits at 617 nm.
  • Chicken Annexin V-Fluorescein, lyophilized, 1 vial, #6643
  • Annexin Reconstitution Buffer, 1X, 1 mL, 1 vial, #6639
  • Annexin Binding Buffer, 10X, 10 mL, 3 bottles, #6640
  • Propidium Iodide, 250 µg/mL, 1 mL, 1 vial, #638

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