Our Anti-VGlut3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N34/34. It detects electric ray, human, mouse and rat VGlut3, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Vesicular glutamate transporter 3 (VGLUT3) or Solute Carrier Family 17 Member 8 is encoded by the gene SLC17A8 and belongs to the vesicular glutamate transporter family. Vglut3 transports glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. Vglut3 is expressed in many tissues including brain, kidney and liver. In brain, it is expressed within glutamatergic neurons in the amygdala, brainstem, cerebral cortex, dorsal root ganglia, dorsal spinal cord, hippocampus, and hypothalamus. Diseases associated with SLC17A8 include Deafness.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N34/34
IgG1
ICC, IHC, WB
Mouse
Slc17a8 Vglut3
65 kDa
Fusion protein amino acids 546-588 (cytoplasmic C-terminus) of rat VGlut3 (accession number NP_714947) produced recombinantly in E. Coli
Drosophila, Human, Mouse, Rat
AB_2187711
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.6
WB: 1:100
IHC: 1:250
ICC: 1:200
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Vesicular glutamate transporter 3 (VGluT3) (Solute carrier family 17 member 8)
Sepehrimanesh, M., et al. 2020. Generation and optimization of highly pure motor neurons from human induced pluripotent stem cells via lentiviral delivery of transcription factors. American journal of physiology. Cell physiology, c771-c780.
Li, H., et al. 2014. Individual synaptic vesicles from the electroplaque of Torpedo californica, a classic cholinergic synapse, also contain transporters for glutamate and ATP. Physiological reports, e00206.
Angioni, L., et al. 2016. Involvement of nigral oxytocin in locomotor activity: A behavioral, immunohistochemical and lesion study in male rats. Hormones and behavior, 23-38.
Fyk-Kolodziej, B., et al. 2015. Dopamine in the auditory brainstem and midbrain: co-localization with amino acid neurotransmitters and gene expression following cochlear trauma. Frontiers in neuroanatomy, 88.
Li, H., et al. 2014. Individual synaptic vesicles from the electroplaque of Torpedo californica, a classic cholinergic synapse, also contain transporters for glutamate and ATP. Physiological reports, e00206.
