Our Anti-Synaptotagmin (Thr202) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat Synaptotagmin (Thr202) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.
Immunostaining of 14 DIV rat cortical neurons labeling synaptotagmin when phosphorylated at Thr202 (cat. p1570-202, 1:400, red) and PSD95 (green). The cortical neurons were fixed with methanol at -20C for 10 minutes. Photo courtesy of Gang Liu.
Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin’s ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ICC, WB
Rabbit
SYT1
62 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202 of rat synaptotagmin, conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
ICC: 1:400
Unconjugated
Specific for endogenous levels of the ~62 kDa synaptotagmin protein phosphorylated at Thr 202. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Thr202
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Fernandez-Chacon, R., Königstorfer, A., Gerber, S.H., García, J., Matos, M.F., Stevens, C.F., Brose, N., Rizo, J., Rosenmund, C. and Südhof, T.C., 2001. Synaptotagmin I functions as a calcium regulator of release probability. Nature, 410(6824), p.41-49. PMID: 11242035
Hilfiker, S., Pieribone, V.A., Nordstedt, C., Greengard, P. and Czernik, A.J., 1999. Regulation of synaptotagmin I phosphorylation by multiple protein kinases. Journal of Neurochemistry, 73(3), pp.921-932. PMID: 10461881
Lee, B.H., Min, X., Heise, C.J., Xu, B.E., Chen, S., Shu, H., Luby-Phelps, K., Goldsmith, E.J. and Cobb, M.H., 2004. WNK1 phosphorylates synaptotagmin 2 and modulates its membrane binding. Molecular Cell, 15(5), pp.741-751. PMID: 15350218
Poskanzer, K.E., Marek, K.W., Sweeney, S.T. and Davis, G.W., 2003. Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo. Nature, 426(6966), p.559-563. PMID: 14634669
Wang, C.T., Lu, J.C., Bai, J., Chang, P.Y., Martin, T.F., Chapman, E.R. and Jackson, M.B., 2003. Different domains of synaptotagmin control the choice between kiss-and-run and full fusion. Nature, 424(6951), p.943-947. PMID: 12931189
Related Products
Recently Viewed
Your research is our top priority
Highly-validated antibodies and reagents from our family of brands.
