Our Anti-Synapsin I (Ser9) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse, rat, and zebrafish Synapsin I (Ser9) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.
Immunostaining of cultured mouse caudate neurons showing synapsin I when phosphorylated at Ser9 (cat. p1560-9, 1:500, red). Cells and photo courtesy of QBMCellScience.
Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Serine 9 is the site on synapsin I that is phosphorylated by cAMP-dependent protein kinase and by calcium calmodulin kinase I (Czernik et al., 1987). Phosphorylation of this site is thought to regulate synaptic vesicle function and neurite outgrowth (Kao et al., 2002).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ICC, WB
Rabbit
SYN1
78 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 of rat synapsin, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Bovine, Human, Xenopus
AB_2492244
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
ICC: 1:500
Unconjugated
Specific for endogenous levels of the ~78 kDa synapsin I doublet protein phosphorylated at Ser9. Also weakly labels the ~55 kDa synapsin II protein which has a similar phosphorylation site to that of Ser9 on synapsin I. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Ser9
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Brain protein 4.1 antibody, SYN 1 antibody, SYN 1a antibody, SYN 1b antibody, SYN I antibody, SYN1 antibody, SYN1_HUMAN antibody, SYN1a antibody, SYN1b antibody, Synapsin 1 antibody, Synapsin I antibody, Synapsin-1 antibody, Synapsin1 antibody, SynapsinI antibody, SYNI antibody
Shulman, Y., et al. 2015. ATP binding to synaspsin IIa regulates usage and clustering of vesicles in terminals of hippocampal neurons. Journal of Neuroscience, 35(3), pp.985-998.
Benleulmi-Chaachoua, A., et al. 2016. Protein interactome mining defines melatonin MT 1 receptors as integral component of presynaptic protein complexes of neurons. Journal of Pineal Research, 60(1), pp.95-108.
Shimomura, S., et al. 2010. Identification of an endogenous substrate of zebrafish doublecortin-like protein kinase using a highly active truncation mutant. The Journal of Biochemistry, 147(5), pp.711-722.
