Our Anti-p38 MAPK (Thr180/Tyr182) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse p38 MAPK (Thr180/Tyr182) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.
Western blot of HeLa cell lysates that had been treated with UV (~254 nm) for 0’ or 30’ showing the specific immunolabeling of the ~39 kDa p38 MAPK protein phosphorylated at Thr180/Tyr182.
The three Mitogen-Activated Protein Kinases (MAPKs) are evolutionarily conserved protein kinases that control a vast array of cellular processes. p38 MAPK is one of these kinases and it is activated by both inflammatory cytokines and by stress (Johnson and Lapadat, 2002; Shi and Gaestel, 2002). The p38 MAPK is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system (Philip and Armstead, 2003; Ying et al., 2002). Like the other MAPKs, p38 is activated by a dual specificity kinase that phosphorylates Thr-180 and Tyr-182 (Lin et al., 1995).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
IHC, WB
Rabbit
MAPK14
39 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr180/Tyr182 of rat p38/MAPK, conjugated to keyhole limpet hemocyanin (KLH).
Human
Bovine, Canine, Chicken, Non-Human Primate, Rat, Zebrafish
AB_2492195
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
IHC: 1:250
Unconjugated
Specific for endogenous levels of the ~39 kDa p38 MAPK protein phosphorylated at Thr180/Tyr182. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Phosphorylated
Thr180, Tyr182
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
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Perrott, K.M., et al. 2017. Apigenin suppresses the senescence-associated secretory phenotype and paracrine effects on breast cancer cells. GeroScience, pp.1-13.
Xu, P., et al. 2017. Parvovirus B19 NS1 protein induces cell cycle arrest at G2-phase by activating the ATR-CDC25C-CDK1 pathway. PLoS pathogens, 13(3), p.e1006266.
