Adult mouse brain membrane (MBM) and transfected cell immunoblot: extracts of MBM and COS cells transiently transfected with Neuroligin-1, -2, -3, -4 and -4* plasmids and probed with N110/29 TC supe. Data courtesy of Marc Bolliger and Tom Sudhof, UTSW (now at Stanford).
Neuroligin3 is encoded by the gene NLGN3. Neuroligin3 is a transmembrane scaffolding protein and cell adhesion molecule. It is involved in cell-cell interactions in neurons and in other cell types. It is expressed in many tissues, including the brain where it is located at the postysynaptic membrane and is involved in regulation of excitatory synapses, synapse formation and synaptic transmission. Mutations in this gene may be associated with autism and Asperger syndrome.
ICC, IHC, WB
Fusion protein amino acids 730-848 (intracellular C-terminus) of rat Neuroligin-3 (also known as gliotactin homolog, accession number AAA97871); Mouse: 99% identity (118/119 amino acids identical); Human: 98% identity (116/119 amino acids identical); ~60% identity with Neuroligin-1; ~40% identity with Neuroligin-2
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Does not cross-react with Neuroligin-1, -2, -4 or -4*
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.