Anti-Microtubule Associated Protein 2 (MAP2) Antibody

10 Citations

Our Anti-Microtubule Associated Protein 2 (MAP2) primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, human, mouse, and rat Microtubule Associated Protein 2 (MAP2) and is total IgY fraction. It is great for use in WB, IHC, ICC.

Immunofluorescence of cortical neuron-glial cell culture from E20 rat labeled with Anti- MAP2 (Cat. 1100-MAP2, red, 1:10,000), and Anti-Tau (green). The blue is DAPI staining of nuclear DNA. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.
Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.
Immunofluorescence of a section of rat brain stem showing colabeled with Anti-MAP2 (Cat. 1100-MAP2, red, 1:5000) and Anti-FOX3 (Cat. 583-FOX3 , green, 1:1000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.
Immunolabeling of mouse hippocampal cultures labeled with anti-MAP2 (Cat. 1100-MAP2, 1:1000, red) and anti-synapsin (Cat. 1925-SYNP , 1:1000, green).
Costaining of the primary brain cells isolated from 18-day embryonic rats (E18). Neurons stained with α-MAP2 (green). Copurified astrocytes stained with α-GFAP (red). Image from publication CC-BY-4.0. PMID: 35652638
Immunostaining of rat hippocampus showing specific immunolabeling of MAP2 (Cat. 1100-MAP2, red, 1:1000), Tau-1 (green), and nuclear DNA labeled with DAPI (blue). Photo courtesy of the Miriam Zamorano, University of Texas Health Science Center at Houston.
Immunolabeling of mouse hippocampal cultures labeled with Anti-GFAP (Cat. 620-GFAP, 1:1000, green) and anti-MAP2 (1100-MAP2, 1:1000, red).
Immunolabeling of mouse cortical cultures labeled with Anti-GFAP (Cat. 620-GFAP, 1:1000, green) and anti-MAP2 (1100-MAP2, 1:1000, red).
Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (Cat. 1435-NES, red, 1:500) and anti-MAP2 antibody (Cat. 1100-MAP2, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
Immunofluorescence of rat cerebellum showing strong synaptic staining of VSNL1 (Cat. : 2145-VSNL1, green) in the molecular layer (ML) and MAP2 (Cat. 1100-MAP2, red, 1:2500). The blue stain is nuclear DNA.
Representative images of anti-MAP2 (ca. 1100-MAP2) immunolabeled rat neurons at 5 DIV of T30 treatment. Image from publication CC-BY-4.0. PMID: 37454238
(A-B). Primary hippocampal neurons were stained with MAP2 (Cat. 1100-MAP2, 1:1000) as a negative control under basal conditions (CTRL) and with picrotoxin treatment (PTX). Image from publication CC-BY-4.0. PMID: 37654026
Day 14 (D14) RGC-iNs stained with a DAPI, TAU, and MAP2 (Cat. 1100-MAP2, 1:1000). Image from publication CC-BY-4.0. PMID: 37773257
Representative 3D view images of paired helical filament (PHF)-tau-treated human brain organoid fractions. The organoids were labeled for MAP2 (Cat. 1100-MAP2, 1:1000, green), NR3A (red), PSD95 (magenta), and DAPI (blue). White arrows show the positions colocalized between PSD95 and NR3A. The dotted circles show the magnified positions. Scale bar = 10 or 3 μm. Image from publication, CC-BY-4.0. PMID: 39396906
Immunofluorescence of cortical neuron-glial cell culture from E20 rat labeled with Anti- MAP2 (Cat. 1100-MAP2, red, 1:10,000), and Anti-Tau (green). The blue is DAPI staining of nuclear DNA. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.
Immunofluorescence of cortical neuron-glial cell culture from E20 rat labeled with Anti- MAP2 (Cat. 1100-MAP2, red, 1:10,000), and Anti-Tau (green). The blue is DAPI staining of nuclear DNA. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.
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Bovine, Human, Mouse, Rat
ICC, IHC, WB
Chicken

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Data Sheet
Trial Size Trial Size

SKU: 1100-MAP2

Volume: 100 µL
Price:
$385.00
Ships: 1-2 business days

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
377732571:1000Agarwal, D, et al. 2023. Human retinal ganglion cell neurons generated by synchronous BMP inhibition and transcription factor mediated reprogramming. NPJ Regenerative medicine, 55.
Immunocytochemistry: Mouse
PMID Dilution Publication
39095513not listedLim, W.L., et al. 2024. CREB-regulated transcription during glycogen synthesis in astrocytes. Scientific Reports, 17942.
37694138not listedSoon, HR, et al. 2023. Seizure enhances SUMOylation and zinc-finger transcriptional repression in neuronal nuclei. iScience, 107707.
34247625 1:500 Khamsing, D., et al. 2021. A role for BDNF-and NMDAR-induced lysosomal recruitment of mTORC1 in the regulation of neuronal mTORC1 activity. Molecular Brain, 14(1), pp.1-20.
27762280 1:2000 Allen, M., et al. 2016. Protease induced plasticity: matrix metalloproteinase-1 promotes neurostructural changes through activation of protease activated receptor 1. Scientific Reports, 6.
24784359 1:20,000 Ho, V.M., et al. 2014. GluA2 mRNA distribution and regulation by miR-124 in hippocampal neurons. Molecular and Cellular Neuroscience , 61, pp.1-12.
Immunocytochemistry: Rat
PMID Dilution Publication
393080631:500Schneeweis, A., et al. 2024. Mass spectrometry identifies tau C-terminal phosphorylation cluster during neuronal hyperexcitation. Journal of Neurochemistry, .
376540261:1000Abdel-Ghani, M, et al. 2023. Plk2 promotes synaptic destabilization through disruption of N-cadherin adhesion complexes during homeostatic adaptation to hyperexcitation. Journal of Neurochemistry, .
37454238not listedGraur, A, et al. 2023. The Human Acetylcholinesterase C-terminal T30 Peptide Activates Neuronal Growth Through Alpha 7 Nicotinic Acetylcholine Receptors and the mTOR Pathway. Scientific Reports, 11434.
35652638not listedNaumov, A.V., et al. 2022. Restriction Checkpoint Controls Bradyzoite Development in Toxoplasma gondii. Microbiology Spectrum, e0070222.

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