Anti-Kv1.2 Potassium Channel Subunit Antibody (L76/36)

Name: Anti-Kv1.2 Potassium Channel Subunit Antibody
Brand: NeuroMab
SKU: 75-314
Price: 337.0 USD
Availability: InStock

Our mouse Kv1.2 potassium channel subunit monoclonal antibody from NeuroMab is produced in-house from clone L76/36. It detects human, mouse, and rat Kv1.2 potassium channel subunit and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Adult rat brain membrane immunoblot.

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-314

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Kv1.2 potassium channel subunit

Scientific Background

Potassium voltage-gated channel subfamily A member 2 (also known as Potassium Voltage-Gated Channel A Member 2 , Shaker-Related Subfamily, Member 2 or Voltage-Gated Potassium Channel Protein Kv1.2, or KCNA2) is a member of the Kv family of potassium channels. Kv1.2 contains six membrane spanning domains and belongs to the delayer rectifier class of potassium channels. Kv2.1 mediates the voltage dependent potassium ion permeability of excitable membranes. Kv1.2 binds PDZ domains of DLG1, DLG2 and DLG4. Kv1.2 is found primarily in the brain (at the axon initial segment, axon preterminals and juxtaparanode domains), central nervous system, but also in the cardiovascular system. Kv2.1 has been implicated in epileptic encephalopathy, early infantile, and episodic ataxia, type 1.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

L76/36

Isotype

IgG2a

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

KCNA2

Molecular Weight

80 kDa

Immunogen

Fusion protein amino acids 428-499 (QYLQVTSCPKIPSSPDLKKSRSASTISKSDYMEIQEGVNNSN EDFREENLKTANCTLANTNYVNITKMLTDV, cytoplasmic C-terminus) of human Kv1.2 (accession number P16389) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315859

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

EIEE32; Potassium voltage-gated channel subfamily A member 2; HK4; NGK1; HUKIV; Voltage-gated K(+ channel HuKIV; KV1.2; Voltage-gated potassium channel HBK5; Voltage-gated potassium channel subunit Kv1.2; RBK2; voltage-gated potassium channel protein Kv1.2; MK2

UniProt ID

P16389
UniProt Summary: Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain and the central nervous system, but also in the cardiovascular system. Prevents aberrant action potential firing and regulates neuronal output. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, KCNA6, KCNA7, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel. Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation of delayed rectifier potassium channels. In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes, making it difficult to assign currents observed in intact tissues to any particular potassium channel family member. Homotetrameric KCNA2 forms a delayed-rectifier potassium channel that opens in response to membrane depolarization, followed by slow spontaneous channel closure. In contrast, a heteromultimer formed by KCNA2 and KCNA4 shows rapid inactivation. Regulates neuronal excitability and plays a role as pacemaker in the regulation of neuronal action potentials. KCNA2-containing channels play a presynaptic role and prevent hyperexcitability and aberrant action potential firing. Response to toxins that are selective for KCNA2-containing potassium channels suggests that in Purkinje cells, dendritic subthreshold KCNA2-containing potassium channels prevent random spontaneous calcium spikes, suppressing dendritic hyperexcitability without hindering the generation of somatic action potentials, and thereby play an important role in motor coordination. Plays a role in the induction of long-term potentiation of neuron excitability in the CA3 layer of the hippocampus. May function as down-stream effector for G protein-coupled receptors and inhibit GABAergic inputs to basolateral amygdala neurons. May contribute to the regulation of neurotransmitter release, such as gamma-aminobutyric acid (GABA). Contributes to the regulation of the axonal release of the neurotransmitter dopamine. Reduced KCNA2 expression plays a role in the perception of neuropathic pain after peripheral nerve injury, but not acute pain. Plays a role in the regulation of the time spent in non-rapid eye movement (NREM) sleep.

NCBI Gene ID

3737

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Anti-Kv1.2 Potassium Channel Subunit Antibody (L76/36)

SKU: 75-314

Product Details

Bulk Order Anti-Kv1.2 Potassium Channel Subunit Antibody

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