Anti-KCC2 Antibody (N1/66)

Name: Anti-KCC2 Antibody
Brand: NeuroMab
SKU: 75-050
Price: 337.0 USD
Availability: InStock

Our mouse KCC2 monoclonal antibody from NeuroMab is produced in-house from clone N1/66. It detects chicken, human, mouse, and rat KCC2 and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Adult rat brain membrane immunoblot.

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Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-050

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

KCC2

Scientific Background

Solute carrier family 12 member 5 is encoded by the gene SLC12A5 and belongs to the SLC12 family of cation-coupled chloride transporters. SLC12A5 is an integral membrane K-Cl cotransporter that mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. Diseases associated with SLC12A5 include Early Infantile Epileptic Encephalopathy 34 and Idiopathic Generalized Epilepsy 14.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N1/66

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Slc12a5 Kcc2 Kiaa1176

Molecular Weight

140 kDa

Immunogen

Fusion protein amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2b (accession number Q63633) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Chicken, Human, Mouse, Rat

Antibody Registry ID

AB_2188800

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

ICC: 1:500

Conjugate

Unconjugated

Specificity

Does not react with KCC1, KCC3 or KCC4

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Electroneutral potassium chloride cotransporter 2; Solute carrier family 12 member 5; Electroneutral potassium-chloride cotransporter 2; Electroneutral potassium-chloride cotransporter 2; Erythroid K Cl cotransporter 2; K-Cl cotransporter 2; Furosemide sensitive K Cl cotransporter; mKCC2; hKCC2; Neuronal K-Cl cotransporter; K-Cl cotransporter 2; KCC 2; KCC2; KIAA1176; Neuronal K Cl cotransporter; Neuronal K-Cl cotransporter; Potassium Chloride Cotransporter; Potassium chloride transporter 5; rKCC2; S12A5; S12A5_HUMAN; SLC12A5; Solute carrier family 12 (potassium chloride transporter member 5; Solute carrier family 12 member 5

UniProt ID

Q63633
UniProt Summary: Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. Involved in the regulation of dendritic spine formation and maturation.

NCBI Gene ID

171373

Shipping

Shipped on ice packs

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Anti-KCC2 Antibody (N1/66)

SKU: 75-050

Product Details

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