Anti-KA2 Kainate Receptor Antibody (N279B/27)

Name: Anti-KA2 Kainate Receptor Antibody
Brand: NeuroMab
SKU: 75-362
Price: 329.0 USD
Availability: InStock

2 Citations

Our Anti-KA2 kainate receptor mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N279B/27. It is KO validated, detects mouse and rat KA2 kainate receptor, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot against crude brain membrane preparations from adult rat (RBM) or wild-type (WT) or KA2 knockout (KO) mice probed with N279B/27 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Anis Contractor (Northwestern University.

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Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-362

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

KA2 kainate receptor

Scientific Background

Glutamate Ionotropic Receptor Kainate Type Subunit 5 is encoded by the gene GRIK5. GRIK5 is a member of the glutamate-gated ion channel (TC 1.A.10.1) family, GRIK5 subfamily. GRIK5 forms functional heteromeric kainate-preferring ionic channels with the subunits encoded by related gene family members. GRIK5 is highly expressed in the cerebral cortex, pyriform cortex, caudate-putamen, hippocampal complex, medial habenulata, and granule cell layer of the cerebellum. GRIK5 is weakly expressed in the globus pallidus, thalamus, colliculi, and the reticular thalamic nucleus. Diseases associated with GRIK5 include Schizophrenia and Autosomal Recessive Non-Syndromic Intellectual Disability.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N279B/27

Isotype

IgG2a

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Grik5

Molecular Weight

110 kDa

Immunogen

Fusion protein amino acids 825-979 (cytoplasmic C-terminus) of rat KA2 kainate receptor (accession number Q63273) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2315855

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with KA1

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Glutamate receptor ionotropic, kainate 5 (GluK5) (Glutamate receptor KA-2) (KA2)

UniProt ID

Q63273

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blot: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
33472038	not listed	Largo-Barrientos, P., et al. 2021. Lowering Synaptogyrin-3 expression rescues Tau-induced memory defects and synaptic loss in the presence of microglial activation.. Neuron, 767-777.
33057002	1:500	Apóstolo, N., et al. 2020. Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer. Nature Communications, 5171.

Western Blot: Mouse
PMID	Dilution	Publication
33472038	not listed	Largo-Barrientos, P., et al. 2021. Lowering Synaptogyrin-3 expression rescues Tau-induced memory defects and synaptic loss in the presence of microglial activation.. Neuron, 767-777.

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Anti-KA2 Kainate Receptor Antibody (N279B/27)

SKU: 75-362

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-KA2 Kainate Receptor Antibody

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