Anti-INPP4b Antibody (N171/17)

Name: Anti-INPP4b Antibody
Brand: NeuroMab
SKU: 75-218
Price: 329.0 USD
Availability: InStock

Our Anti-INPP4b mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N171/17. It detects human INPP4b, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Immunoblot of Inpp4b+/+ or Inpp4b−/− mouse embryonic fibroblasts treated with vehicle or apilimod 10 nM for 48 h and assessed for protein levels of Inpp4b, LC3, and Beta actin as loading control. Image from publication CC-BY-4.0. PMID: 35760104

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged INPP4b or untagged Kv2.1 plasmid and probed with N171/17 (left) and N86/8 (right) TC supe.

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Species Reactivity

Human, Mouse

Applications

ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

SKU: 75-218

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

INPP4b

Target Description

Inositol polyphosphate 4-phosphatase type II is encoded by the gene INPP4B. INPP4B is a member of the inositol 3,4-bisphosphate 4-phosphatase family. INPP4B is an enzyme involved in phosphatidylinositol pathways. This enzyme catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-triphosphate, and inositol 3,4-triphosphate. The lipid phosphatase activity is critical for tumor suppressor function. INPP4B activity modulates cell cycle progression and cell survival. INPP4B is widely expressed with the highest levels occuring in the skeletal muscle and heart. No disorders were found to be associated with INPP4B.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N171/17

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

INPP4B

Molecular Weight

100 kDa

Antigen

Fusion protein amino acids 395-518 of human INPP4b (accession number O15327) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse

Antibody Registry ID

AB_11001557

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with INPP4a

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Type II inositol 3,4-bisphosphate 4-phosphatase (EC 3.1.3.66) (Inositol polyphosphate 4-phosphatase type II)

UniProt ID

O15327

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
35760104	not listed	Saffi, G.T., et al. 2022. Inhibition of Lipid Kinase PIKfyve Reveals a Role for Phosphatase Inpp4b in the Regulation of PI(3)P-Mediated Lysosome Dynamics Through VPS34 Activity. The Journal of Biological Chemistry, 102187.

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Anti-INPP4b Antibody (N171/17)

SKU: 75-218

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-INPP4b Antibody

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