Anti-Fascin (clone 55K2) Antibody (55K2)

Name: Anti-Fascin (clone 55K2) Antibody
Brand: PhosphoSolutions
SKU: FM2651
Price: 285.0 USD
Availability: InStock

4 Citations

Our Fascin (clone 55K2) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Fascin (clone 55K2) and is Protein A purified. It is great for use in WB, ICC, IHC.

Western blot analysis of human HeLa cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Fascin (clone 55K2) (lanes 1 & 2) and anti-Fascin (Ser-39) (lanes 3 & 4).

Immunocytochemical labeling of Fascin in aldehyde fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Fascin (FM2651). The antibody was detected using goat anti-mouse DyLight® 594.

Sea urchin embryos were injected with control miR-124 LNA inhibitor or miR-31 LNA inhibitor and immunolabeled with Fascin antibody (Cat. FM2651, green) and tubulin antibody (magenta) followed by DAPI counterstain for DNA (blue). Image from publication CC-BY-4.0. PMID: 37398341

Embryos were immunolabeled with Fascin antibodies (Cat. FM2651, 1:100) pre-adsorbed with lysate from bacteria expressing HA-tagged Fascin or human Rab35-GST. Embryos immunolabeled with pre-adsorbed Fascin antibodies have decreased Fascin (shown in green) compared to embryos immunolabeled with non-pre-adsorbed Fascin antibody. Image from publication CC-BY-4.0. PMID: 37033709

Western blot analysis of human HeLa cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Fascin (clone 55K2) (lanes 1 & 2) and anti-Fascin (Ser-39) (lanes 3 & 4).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

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Data Sheet

SKU: FM2651

Volume: 100 µL

Variant

Price:

$285.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Fascin (clone 55K2)

Scientific Background

Fascin is an actin filament bundling protein localized to lamellipodia and filopodia where it has important roles in cell motility. Regulation of fascin occurs through PKC-mediated phosphorylation of Ser-39 in the F-actin binding site. Cell permeant peptides that block PKC phosphorylation of Ser-39 increase cell migration, while peptides that block fascin binding to F-actin alter lamellipodial morphology and cause aberrant cell motility. Studies using RNA interference of fascin show that fibroblasts have reduced number and abnormal morphology of filopodia, while Ser-39 phosphorylation status may determine filopodial frequency. In Drosophila neurons, fascin deficiency causes alterations in actin filaments and leads to abnormal morphology of developing neurons. Thus, fascin is a critical element of actin-based motility in various cell types.

Format

Protein A Purified

Clonality

Monoclonal

Clone

55K2

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

FSCN1

Molecular Weight

Immunogen

Clone (55K2) was generated from full-length human fascin purified from HeLa cells. This sequence has high homology to rat and mouse fascin.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IHC: 1:100
ICC: 1:100
IP: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 55 kDa* band corresponding to fascin on SDS-PAGE immunoblots of human A431 and HeLa, as well as mouse C2C12 and rat PC12.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

p55

UniProt ID

Q16658

Shipping

Blue Ice

Product Specific References for Applications and Species

Immunohistochemistry: Sea Urchin
Proximity Ligation Assay: Sea Urchin
Western Blot: Mouse

Immunohistochemistry: Sea Urchin
PMID	Dilution	Publication
37398341	not listed	Remsburg, C, et al. 2023. miR-31-mediated local translation at the mitotic spindle is important for early development. Research square, .
37033709	1:100	Testa, MD, et al. 2023. The actin bundling protein Fascin is important for proper early development in Strongylocentrotus purpuratus embryos. microPublication Biology, .

Proximity Ligation Assay: Sea Urchin
PMID	Dilution	Publication
39250531	1:100	Remsburg, CM, et al. 2024. miR-31-mediated local translation at the mitotic spindle is important for early development. Development, .

Western Blot: Mouse
PMID	Dilution	Publication
28369442	not listed	Elizondo, DM, et al. 2017. CD40-CD40L cross-talk drives fascin expression in dendritic cells for efficient antigen presentation to CD4+ T cells. International immunlogy, 121-131.

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Anti-Fascin (clone 55K2) Antibody (55K2)

SKU: FM2651

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Fascin (clone 55K2) Antibody

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