Anti-EB3 (central region) Antibody

Name: Anti-EB3 (central region) Antibody
Brand: PhosphoSolutions
SKU: EP5121
Price: 275.0 USD
Availability: InStock

Our EB3 (central region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat EB3 (central region) and is antigen affinity purified. It is great for use in WB.

Western blot analysis of adult mouse brain untreated (lanes 1, 3, 5, & 7) or treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with rat monoclonal anti-EB3 (EM5091) (lanes 1 & 2), and rabbit polyclonals anti-EB3 (Ser-162) (lanes 3 & 4), anti-EB3 (Ser-176) (lanes 5 & 6), and anti-EB3 (a.a. 171-182) (lanes 7 & 8).

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

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SKU: EP5121

Volume: 100 µL

Variant

Price:

$275.00

Quantity:

Product Details

Target

EB3 (central region)

Target Description

The microtubule (MT) plus-end is a crucial site for the regulation of MT dynamics and interactions by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions with organelles and macromolecular complexes. The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases as well as various regulatory and adaptor proteins. The CLIP-associated protein (CLASP) family includes CLASP1 and CLASP2 proteins, which are expressed as long (α) and short (β) isoforms. Thse +TIPs conatin an N-terminal TOG domain, multiple TOG-like domains, and a basic and serine-rich motif (SxIP). The TOG domain facilitates interaction with tubulin dimers, while the SxIP motif promotes interaciton with EB1 and MTs. A C-terminal domain is involved in interaction with CLIPs, as well as several other proteins. CLASPs are MT stabilizing fators that localize to mitotic spindles, kinetochores, and the midbody. CLASPs are important for cell division, and may regulate cell migration and neuronal growth cone motility.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

MAPRE3

Molecular Weight

Antigen

EB3 synthetic peptide (coupled to KLH) corresponding to amino acid residues from the central region of human EB3. This peptide sequence is highly conserved in mouse and rat EB3, and has low homology to other EB family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects a 32 kDa* protein corresponding to the apparent molecular mass of EB3 on SDS-PAGE immunoblots of mouse brain and human recombinant full length EB3 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Microtubule RP/EB, MAPRE3, APC, EB3, End-binding. RP3

UniProt Details

UniProt (Human): Q9UPY8
UniProt (Immunogen Species): Q9UPY8

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Anti-EB3 (central region) Antibody

SKU: EP5121

Product Details

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