Anti-Centromere Protein Antibody

305 Citations

Our Anti-Centromere proteins human polyclonal primary antibody from Antibodies Incorporated is produced in-house. It detects Centromere proteins, and is antisera. It is great for use in IHC, ICC.

A microscopy image of a cell from BJ hTERT cell line treated with aphidicolin to induce genomic instability. Immunolabeling of the cell positively labelling CREST/Centromere Protein (red, 1:500, Cat No. 15-234) and labelling DNA with DAPI. The anaphase cell has a segregation error which lacks centromeric staining unlike the mass of properly segregating chromosomes. Image kindly provided by Nadeem Shaikh and Sarah McClelland from Barts Cancer Institute, Queen Mary University of London.
Confocal image of kinetochore-microtubule attachments in mouse control (CON) and IMQ treated oocytes where kinetochores are dyed purple by CREST antibody, microtubules are stained green by the anti-tubulin antibody. Chromosomes are dyed blue by Hoechst 33342.Image from publication CC-BY-4.0. PMID: 35589679
Immunofluorescence showing SCP3 (green) and the co-localization of VCL (red) and CREST-labeling centromeres (blue) on squashed mouse spermatocytes. Image from publication CC-BY-4.0. PMID: 35805097
Immunofluorescence of control and nivalenol (NIV) treated mouse oocytes where α-tubulin is labeled green, centromeres are labeled red and DNA is dyed blue by Hoechst. Image from publication CC-BY-4.0. PMID: 35746834
Immunostaining showing that there is no difference for centromere intensity between mouse WT and Fbxw24-KO germ cells, meanwhile SYCP3 intensity significantly increased in Fbxw24-KO germ cells. DNA in blue, SYCP3 in red, centromere in green. Image from publication CC-BY-4.0. PMID: 35858239
Representative maximum z-projections of in situ chromosome spreads from a mouse euploid egg (kinetochores (CREST), green or gray scale) (DNA, blue). Image from publication CC-BY-4.0. PMID: 35810327
Immunofluorescence of HeLa Kyoto cells transiently expressing different Sgo1-GFP constructs (Sgo1-GFP, Sgo1Nmut-GFP, Sgo14A-GFP, or Sgo1Nmut/4A-GFP) and depleted of endogenous Sgo1 using siRNA oligonucleotides showing endogenous Borealin and ACA. DAPI was used for DNA staining. Image from publication CC-BY-4.0. PMID: 35776132
Uninjected, Control, CBS-KD human oocytes cultured in M16 medium for 8 h to MI for immunofluorescence with Mitotic arrest deficiency 1 (MAD1) (red) and human anti-centromere antibody (CREST) (green). Image from publication CC-BY-4.0. PMID: 36053797
Human oocytes in Uninjected, Control, CBS-KD groups were cultured in M16 medium for 8 h to MI and then incubated on ice for 5 min and processed for immunofluorescence with acetylated α-tubulin (red) and CREST antibody (green). Image from publication CC-BY-4.0. PMID: 36053797
Representative image of RPE1 cell with an acentric micronucleus. CREST antibody was used to stain for presence of centromeric proteins. Image from publication CC-BY-4.0. PMID:36266663
Immunofluorescence images of porcine oocytes shortly after NEBD (z-projections, example 1: 14 sections, example 2: 11 sections every 0.19 µm) and after chromosome cluster formation (z-projection, 14 sections every 0.19 µm). F-actin (phalloidin; white); kinetochores (ACA)(cat. 15-234, 1:250; cyan); DNA (Hoechst, magenta,). Image from publication CC-BY-4.0. PMID:36732633
Control and Pak2-KD metaphase mouse oocytes were labeled with CREST antibody for kinetochores (cat. 15-234, 1:300; purple), anti-tubulin antibody for microtubules (green), and Hoechst 33342 for chromosomes (blue). Image from publication CC-BY-4.0. PMID:36813765
Representative images of the morphology of K-M attachments in MI oocytes from both control and BBP-exposed group. α-tubulin (gray); CREST (cat. 15-234, 1:200; red); DNA (blue). Image from publication CC-BY-4.0. PMID:36756972
Localization of telomeric sequences in Thylamys elegans and Macropus eugenii. Spread spermatocytes were labeled with antibodies against SYCP3 (blue), centromeric proteins (cat. 15-234, 1:50; red) and telomeric sequences (green) in (D) and (H). Image from publication CC-BY-4.0. PMID:37181752
Costaining of chromosomes (DAPI, blue) and centromeres (cat. 15-234, 1:250; green) in representative nuclei of metaphase short-term cultures, established from Nf2KO-Setd2KO-Trp53KO-4q9p21 tumor-bearing mice. Image from publication CC-BY-4.0. PMID:37365326
Representative immunofluorescence images of RioK1 at kinetochores (CREST) (cat. 15-234, 1:50) in prometaphase and metaphase cells. Image from publication CC-BY-4.0. PMID:37263996
Confocal imaging and quantification of Plk1 signal intensities at centrosomes and kinetochores (cat. 15-234) after treating HeLa cells with DMSO or 10 μM of Allopole for 2 h. Arrowheads, centrosomes; arrows, misaligned chromosomes. Signal intensities were quantified from both centrosome signals and randomly chosen three to four kinetochore signals per cell for a total of greater than or equal to 18 cells/experiment. Image from publication CC-BY-4.0. PMID:37603740
Pachytene and pachytene-like spermatocytes of A. maximowiczii, MAX (A–C), A. evoronensis “Argi” × A. evoronensis “Evoron”, EVA × EVE (D–F), A. mujanensis × A. maximowiczii, MUJ × MAX (G–I), A. evoronensis “Argi” × A. mujanensis, EVA × MUJ (J–L), and A. evoronensis “Argi” × A. maximowiczii, EVA × MAX (M–O) after immunolocalization of SYCP3, SYCP1, MLH1, γH2A.X, and centromeric proteins (ACA, cat. 15-234, 1:100). Arrowheads show asynapsed SC regions of the autosomes. CC-BY-4.0. PMID:37263996
Representative images of chromosome spreading (cat. 15-234, 1:200) of euploid and aneuploid mouse oocytes. Image from publication CC-BY-4.0. PMID:37386516
RPE-1 cells expressing H2B-GFP and α-Tub-mRFP (magenta) were treated as indicated, fixed and stained for centromeres (ACA) (cat. 15-234, 1:400; white) and DNA (DAPI, blue). BI2536 was added at the IC30 concentration (5 nM). Representative cells are shown, with their measured spindle lengths and centromere dispersion ratios (r). Image from publication CC-BY-4.0. PMID:37639469
RPE1 53BP1‐V5 cells were either left untreated (async = asynchronous) or synchronized in prometaphase in medium containing nocodazole, fixed immediately (M = mitosis) or after 2, 4, or 6 h and co‐stained with V5 and CREST (cat. 15-234, 1:500). Image from publication CC-BY-4.0. PMID:37888778
C3a-peptide treatment does not induce chromosome aneuploidy of mouse oocytes. Representative images of MII oocyte immunofluorescence stained with DAPI (blue) and anti-CREST (cat. 15-234, 1:500; magenta) antibodies showing euploidy and aneuploidy in the oocytes, respectively (left). Image from publication CC-BY-4.0. PMID:38066646
Pachytene spermatocytes of linnet (A), bullfinch (B), siskin (C), and goldfinch (D) after immunolocalization of SYCP3 (red), MLH1 (green) and centromere proteins (blue). Arrowheads indicate metapolycentromeres and GRC. Image from publication CC-BY-4.0. PMID:38066976
Immunostaining with anti-centromere antibody ACA to show centromeres in detached chromosome ends in Zfp541 KO pachytene spermatocytes. Image from publication CC-BY-4.0. PMID:37921559
A microscopy image of a cell from BJ hTERT cell line treated with aphidicolin to induce genomic instability. Immunolabeling of the cell positively labelling CREST/Centromere Protein (red, 1:500, Cat No. 15-234) and labelling DNA with DAPI. The anaphase cell has a segregation error which lacks centromeric staining unlike the mass of properly segregating chromosomes. Image kindly provided by Nadeem Shaikh and Sarah McClelland from Barts Cancer Institute, Queen Mary University of London.
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A microscopy image of a cell from BJ hTERT cell line treated with aphidicolin to induce genomic instability. Immunolabeling of the cell positively labelling CREST/Centromere Protein (red, 1:500, Cat No. 15-234) and labelling DNA with DAPI. The anaphase cell has a segregation error which lacks centromeric staining unlike the mass of properly segregating chromosomes. Image kindly provided by Nadeem Shaikh and Sarah McClelland from Barts Cancer Institute, Queen Mary University of London.
Confocal image of kinetochore-microtubule attachments in mouse control (CON) and IMQ treated oocytes where kinetochores are dyed purple by CREST antibody, microtubules are stained green by the anti-tubulin antibody. Chromosomes are dyed blue by Hoechst 33342.Image from publication CC-BY-4.0. PMID: 35589679
Immunofluorescence showing SCP3 (green) and the co-localization of VCL (red) and CREST-labeling centromeres (blue) on squashed mouse spermatocytes. Image from publication CC-BY-4.0. PMID: 35805097
Immunofluorescence of control and nivalenol (NIV) treated mouse oocytes where α-tubulin is labeled green, centromeres are labeled red and DNA is dyed blue by Hoechst. Image from publication CC-BY-4.0. PMID: 35746834
Immunostaining showing that there is no difference for centromere intensity between mouse WT and Fbxw24-KO germ cells, meanwhile SYCP3 intensity significantly increased in Fbxw24-KO germ cells. DNA in blue, SYCP3 in red, centromere in green. Image from publication CC-BY-4.0. PMID: 35858239
Representative maximum z-projections of in situ chromosome spreads from a mouse euploid egg (kinetochores (CREST), green or gray scale) (DNA, blue). Image from publication CC-BY-4.0. PMID: 35810327
Immunofluorescence of HeLa Kyoto cells transiently expressing different Sgo1-GFP constructs (Sgo1-GFP, Sgo1Nmut-GFP, Sgo14A-GFP, or Sgo1Nmut/4A-GFP) and depleted of endogenous Sgo1 using siRNA oligonucleotides showing endogenous Borealin and ACA. DAPI was used for DNA staining. Image from publication CC-BY-4.0. PMID: 35776132
Uninjected, Control, CBS-KD human oocytes cultured in M16 medium for 8 h to MI for immunofluorescence with Mitotic arrest deficiency 1 (MAD1) (red) and human anti-centromere antibody (CREST) (green). Image from publication CC-BY-4.0. PMID: 36053797
Human oocytes in Uninjected, Control, CBS-KD groups were cultured in M16 medium for 8 h to MI and then incubated on ice for 5 min and processed for immunofluorescence with acetylated α-tubulin (red) and CREST antibody (green). Image from publication CC-BY-4.0. PMID: 36053797
Representative image of RPE1 cell with an acentric micronucleus. CREST antibody was used to stain for presence of centromeric proteins. Image from publication CC-BY-4.0. PMID:36266663
Immunofluorescence images of porcine oocytes shortly after NEBD (z-projections, example 1: 14 sections, example 2: 11 sections every 0.19 µm) and after chromosome cluster formation (z-projection, 14 sections every 0.19 µm). F-actin (phalloidin; white); kinetochores (ACA)(cat. 15-234, 1:250; cyan); DNA (Hoechst, magenta,). Image from publication CC-BY-4.0. PMID:36732633
Control and Pak2-KD metaphase mouse oocytes were labeled with CREST antibody for kinetochores (cat. 15-234, 1:300; purple), anti-tubulin antibody for microtubules (green), and Hoechst 33342 for chromosomes (blue). Image from publication CC-BY-4.0. PMID:36813765
Representative images of the morphology of K-M attachments in MI oocytes from both control and BBP-exposed group. α-tubulin (gray); CREST (cat. 15-234, 1:200; red); DNA (blue). Image from publication CC-BY-4.0. PMID:36756972
Localization of telomeric sequences in Thylamys elegans and Macropus eugenii. Spread spermatocytes were labeled with antibodies against SYCP3 (blue), centromeric proteins (cat. 15-234, 1:50; red) and telomeric sequences (green) in (D) and (H). Image from publication CC-BY-4.0. PMID:37181752
Costaining of chromosomes (DAPI, blue) and centromeres (cat. 15-234, 1:250; green) in representative nuclei of metaphase short-term cultures, established from Nf2KO-Setd2KO-Trp53KO-4q9p21 tumor-bearing mice. Image from publication CC-BY-4.0. PMID:37365326
Representative immunofluorescence images of RioK1 at kinetochores (CREST) (cat. 15-234, 1:50) in prometaphase and metaphase cells. Image from publication CC-BY-4.0. PMID:37263996
Confocal imaging and quantification of Plk1 signal intensities at centrosomes and kinetochores (cat. 15-234) after treating HeLa cells with DMSO or 10 μM of Allopole for 2 h. Arrowheads, centrosomes; arrows, misaligned chromosomes. Signal intensities were quantified from both centrosome signals and randomly chosen three to four kinetochore signals per cell for a total of greater than or equal to 18 cells/experiment. Image from publication CC-BY-4.0. PMID:37603740
Pachytene and pachytene-like spermatocytes of A. maximowiczii, MAX (A–C), A. evoronensis “Argi” × A. evoronensis “Evoron”, EVA × EVE (D–F), A. mujanensis × A. maximowiczii, MUJ × MAX (G–I), A. evoronensis “Argi” × A. mujanensis, EVA × MUJ (J–L), and A. evoronensis “Argi” × A. maximowiczii, EVA × MAX (M–O) after immunolocalization of SYCP3, SYCP1, MLH1, γH2A.X, and centromeric proteins (ACA, cat. 15-234, 1:100). Arrowheads show asynapsed SC regions of the autosomes. CC-BY-4.0. PMID:37263996
Representative images of chromosome spreading (cat. 15-234, 1:200) of euploid and aneuploid mouse oocytes. Image from publication CC-BY-4.0. PMID:37386516
RPE-1 cells expressing H2B-GFP and α-Tub-mRFP (magenta) were treated as indicated, fixed and stained for centromeres (ACA) (cat. 15-234, 1:400; white) and DNA (DAPI, blue). BI2536 was added at the IC30 concentration (5 nM). Representative cells are shown, with their measured spindle lengths and centromere dispersion ratios (r). Image from publication CC-BY-4.0. PMID:37639469
RPE1 53BP1‐V5 cells were either left untreated (async = asynchronous) or synchronized in prometaphase in medium containing nocodazole, fixed immediately (M = mitosis) or after 2, 4, or 6 h and co‐stained with V5 and CREST (cat. 15-234, 1:500). Image from publication CC-BY-4.0. PMID:37888778
C3a-peptide treatment does not induce chromosome aneuploidy of mouse oocytes. Representative images of MII oocyte immunofluorescence stained with DAPI (blue) and anti-CREST (cat. 15-234, 1:500; magenta) antibodies showing euploidy and aneuploidy in the oocytes, respectively (left). Image from publication CC-BY-4.0. PMID:38066646
Pachytene spermatocytes of linnet (A), bullfinch (B), siskin (C), and goldfinch (D) after immunolocalization of SYCP3 (red), MLH1 (green) and centromere proteins (blue). Arrowheads indicate metapolycentromeres and GRC. Image from publication CC-BY-4.0. PMID:38066976
Immunostaining with anti-centromere antibody ACA to show centromeres in detached chromosome ends in Zfp541 KO pachytene spermatocytes. Image from publication CC-BY-4.0. PMID:37921559

Hamster, Human, Mouse, Rat
ICC, IHC
Human

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Data Sheet

SKU: 15-234

Volume: 1 mL
Price:
$155.00
Ships: 1-2 business days

Product Specific References for Applications and Species

Chromosome Allignment Assays: Mouse
PMID Dilution Publication
36260995not listedFrancois, L., et al. 2022. BCAT1 Redox Function Maintains Mitotic Fidelity. Cell Reports, 111524.
Chromosome Spread: Bovine
PMID Dilution Publication
397471421:100Hashimoto, S, et al. 2025. Nicotinamide mononucleotide boosts the development of bovine oocyte by enhancing mitochondrial function and reducing chromosome lagging. Scientific Reports, 310.
Chromosome Spread: Human
PMID Dilution Publication
398381941:100Zheng, S, et al. 2025. High CDC20 levels increase sensitivity of cancer cells to MPS1 inhibitors. EMBO Reports, .
Chromosome Spread: Mouse
PMID Dilution Publication
399733741:200Wang, G, et al. 2025. Testis-Specific PDHA2 Is Required for Proper Meiotic Recombination and Chromosome Organisation During Spermatogenesis. Cell Proliferation, e70003.
396577881:100Lee, C, et al. 2024. Novel BRCA1-PLK1-CIP2A axis orchestrates homologous recombination-mediated DNA repair to maintain chromosome integrity during oocyte meiosis. Nucleic Acids Research, .
Immunocytochemistry: Bird
PMID Dilution Publication
35389062not listedSotelo-Muñoz, M., et al. 2022. Germline-Restricted Chromosome Shows Remarkable Variation in Size Among Closely Related Passerine Species. Chromosoma, 77-86.
319744271:100Malinovskaya, L.P., et al. 2020. Germline-restricted chromosome (GRC) in the sand martin and the pale martin (Hirundinidae, Aves): synapsis, recombination and copy number variation. Scientific Reports, 1058.
347599621:100Torgasheva, A., et al. 2018. Germline-Restricted Chromosome (GRC) in Female and Male Meiosis of the Great Tit (Parus major, Linnaeus, 1758). Frontiers in Genetics, 768056.
Immunocytochemistry: Hamster
PMID Dilution Publication
391474431:1 (supe)Amadio, F., et al. 2024. Di(2-ethylexyl) phthalate and chromosomal damage: Insight on aneugenicity from the cytochalasin-block micronucleus assay. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 503791.
296935871:70Bikchurina, Tatiana I., et al. 2018. Chromosome Synapsis and Recombination in Male-Sterile and Female-Fertile Interspecies Hybrids of the Dwarf Hamsters ( Phodopus, Cricetidae). Genes, 227.
Immunocytochemistry: Human
PMID Dilution Publication
399299861:1Udroiu, I, et al. 2025. Low-intensity pulsed ultrasound induces multifaced alterations in chromosome segregation, cytoskeletal filaments and cell junctions. Scientific Reports, 4964.
39893637not listedGraham, E, et al. 2025. The homologous recombination factors BRCA2 and PALB2 interplay with mismatch repair pathways to maintain centromere stability and cell viability. Cell Reports, 115259.
39763034not listedZhang, Y, et al. 2025. EHMT2-mediated R-loop formation promotes the malignant progression of prostate cancer via activating Aurora B. Clinical and Translational Medicine, e70164.
397157661:50Bellou, E, et al. 2024. Chromosome architecture and low cohesion bias acrocentric chromosomes towards aneuploidy during mammalian meiosis. Nature Communications, 10713.
39677017not listedWasnik, N, et al. 2024. Quantitative Analysis of Kinetochore Protein Levels and Inter-Kinetochore Distances in Mammalian Cells During Mitosis. Bio-Protocol, e5132.
396042141:1000Ishikawa, Y, et al. 2024. Fibrous corona is reduced in cancer cell lines that attenuate microtubule nucleation from kinetochores. Cancer Science, .
396107071:100Serpico, A.F., et al. 2024. Chromosome alignment and Kif18A action rely on spindle-localized control of Cdk1 activity. Frontiers in Cell and Developmental Biology, 1490781.
395139101:400Falke, K, et al. 2024. Old Passengers as New Drivers: Chromosomal Passenger Proteins Engage in Translesion Synthesis. Cells, 1804.
39208097not listedMartin, S., et al. 2024. A p62-dependent rheostat dictates micronuclei catastrophe and chromosome rearrangements. Science, eadj7446.
391988481:1000Zhu, Y., et al. 2024. Large-scale copy number alterations are enriched for synthetic viability in BRCA1/BRCA2 tumors. Genome Medicine, 108.
391788431:300Ly, J., et al. 2024. A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo development. Current Biology, .
391721431:20Kolar, N., et al. 2024. Genotoxicity of selected cannabinoids in human lymphoblastoid TK6 cells. Archives of Toxicology, .
39152119not listedGalán-Vidal, J., et al. 2024. Sumo-regulatory SENP2 controls the homeostatic squamous mitosis-differentiation checkpoint. Cell Death & Disease, 596.
384785551:25Sutanto, R, et al. 2024. The oncogene cyclin D1 promotes bipolar spindle integrity under compressive force. PloS One, e0296779.
384478821:20Naso, FD, et al. 2024. AurkA/TPX2 co-overexpression in nontransformed cells promotes genome instability through induction of chromosome mis-segregation and attenuation of the p53 signalling pathway. Biochimica et Biophysica Acta, 167116.
384242311:700Lakshmi, RB, et al. 2024. CKAP5 stabilizes CENP-E at kinetochores by regulating microtubule-chromosome attachments. EMBO Reports, 1909-1935.
378887781:500Burigotto, M, et al. 2023. PLK1 promotes the mitotic surveillance pathway by controlling cytosolic 53BP1 availability. EMBO Reports, e57234.
37788673not listedJoo, YK, et al. 2023. ATR promotes clearance of damaged DNA and damaged cells by rupturing micronuclei. Molecular Cell, 3642-3658.e4.
376394691:400Normandin, K, et al. 2023. Genetic enhancers of partial PLK1 inhibition reveal hypersensitivity to kinetochore perturbations. PLoS Genetics, e1010903.
37603740not listedPark, JE, et al. 2023. Specific inhibition of an anticancer target, polo-like kinase 1, by allosterically dismantling its mechanism of substrate recognition. Proceedings of the National Academy of Sciences of the United States of America, e2305037120.
372639961:50Smurova, K, et al. 2023. Rio1 Downregulates Centromeric RNA Levels to Promote the Timely Assembly of Structurally Fit Kinetochores. Nature Communications, 3172.
37163376not listedTrier, I, et al. 2023. ATR Protects Centromere Identity by Promoting DAXX Association with PML Nuclear Bodies. Cell Reports, 112495.
371009001:200Tsang, M.J., et al. 2023. Alternative CDC20 Translational Isoforms Tune Mitotic Arrest Duration. Nature, 154-161.
370630651:250Tovini, L., et al. 2023. Targeted Assembly of Ectopic Kinetochores to Induce Chromosome-Specific Segmental Aneuploidies. The EMBO Journal, e111587.
36917609not listedPavani, M., et al. 2023. Triap1 Upregulation Promotes Escape from Mitotic-Slippage-Induced G1 Arrest. Cell Reports, 112215.
36897279not listedSobajima, T., et al. 2023. PP6 Regulation of Aurora A-TPX2 Limits NDC80 Phosphorylation and Mitotic Spindle Size. The Journal of Cell Biology, .
367326331:250Wang, Y., et al. 2023. Actin-driven chromosome clustering facilitates fast and complete chromosome capture in mammalian oocytes. Nature Cell Biology, .
36658409not listedSiouda, M., et al. 2023. Chromodomain on Y-like 2 (CDYL2) Implicated in Mitosis and Genome Stability Regulation via Interaction with CHAMP1 and POGZ. Cellular and Molecular Life Sciences, 47.
365301671:200Clarke, M.N., et al. 2022. Adaptation to High Rates of Chromosomal Instability and Aneuploidy Through Multiple Pathways in Budding Yeast. The EMBO Journal, e111500.
36450246not listedShindo, N., et al. 2022. Autocleavage of separase suppresses its premature activation by promoting binding to cyclin B1. Cell Reports, 111723.
36415827not listedBoukaba, A., et al. 2022. Mapping Separase-Mediated Cleavage In Situ. NAR Genomics And Bioinformatics, lqac085.
36395767not listedHayward, D., et al. 2022. MPS1 localizes to end-on microtubule-attached kinetochores to promote microtubule release. Current Biology, 5200-5208.
36266663not listedShaikh, N., et al. 2022. Replication Stress Generates Distinctive Landscapes of DNA Copy Number Alterations and Chromosome Scale Losses. Genome Biology, 223.
36053797not listedCao, Y., et al. 2022. Cystathionine β-synthase is Required for Oocyte Quality by Ensuring Proper Meiotic Spindle Assembly. Cell Proliferation, e13322.
359294561:300Dale, K.L., et al. 2022. Modest Increase of KIF11 Expression Exposes Fragilities in the Mitotic Spindle, Causing Chromosomal Instability. Journal of Cell Science, .
358306141:100Navarro, A.P., et al. 2022. Dynamic Cell Cycle-Dependent Phosphorylation Modulates CENP-L-CENP-N Centromere Recruitment. Molecular Biology of the Cell, mbcE22060239.
356532961:500Niedzialkowska, E., et al. 2022. Tip60 acetylation of histone H3K4 temporally controls chromosome passenger complex localization. Molecular Biology of the Cell, br15.
356385751:500Schrock, M.S., et al. 2022. MKLP2 functions in early mitosis to ensure proper chromosome congression. Journal of Cell Science, jcs259560.
355252441:1000Pesenti, M.E., et al. 2022. Structure of the human inner kinetochore CCAN complex and its significance for human centromere organization. Molecular cell, 2113-2131.
35167828not listedKoranne, R., et al. 2022. C9ORF78 partially localizes to centromeres and plays a role in chromosome segregation. Experimental cell research, 113063.
351414991:2000Yamada, C., et al. 2022. TORC1 inactivation promotes APC/C-dependent mitotic slippage in yeast and human cells. iScience, 103675.
35081344not listedSerpico, A.F., et al. 2022. Compartmentalized control of Cdk1 drives mitotic spindle assembly. Cell Reports, 110305.
347895121:100Mammel, A.E., et al. 2022. Chromosome length and gene density contribute to micronuclear membrane stability. Life Science Alliance, e202101210.
34926718not listedLaine, L.J., et al. 2021. VTT-006, an anti-mitotic compound, binds to the Ndc80 complex and suppresses cancer cell growth in vitro. Oncoscience, .
345185271:1600Bakhoum, M.F., et al. 2021. Loss of polycomb repressive complex 1 activity and chromosomal instability drive uveal melanoma progression. Nature Communications, 5402.
343522231:100Ippolito, M.R., et al. 2021. Gene copy-number changes and chromosomal instability induced by aneuploidy confer resistance to chemotherapy. Developmental Cell, 2440-2454.
343423531:500Herlihy, C.P., et al. 2021. Suv420 enrichment at the centromere limits Aurora B localization and function. Journal of Cell Science, jcs249763.
343133101:100Yu, B., et al. 2021. SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A. Journal of Cell Science, jcs249771.
34010649not listedPangou, E., et al. 2021. A PKD-MFF signaling axis couples mitochondrial fission to mitotic progression. Cell Reports, 109129.
339886771:3000Iemura, K., et al. 2021. Chromosome oscillation promotes Aurora A–dependent Hec1 phosphorylation and mitotic fidelity. Journal of Cell Biology, e202006116.
Immunocytochemistry: Kangaroo
PMID Dilution Publication
387278081:25Chong, MK, et al. 2024. Chromosome size-dependent polar ejection force impairs mammalian mitotic error correction. The Journal of Cell Biology, .
Immunocytochemistry: Lizard
PMID Dilution Publication
324574931:500Spangenberg, V., et al. 2020. Cytogenetic mechanisms of unisexuality in rock lizards. Scientific Reports, 8697.
Immunocytochemistry: Mouse
PMID Dilution Publication
39947176not listedDudka, D, et al. 2025. Adaptive evolution of CENP-T modulates centromere binding. Current Biology, .
39921465not listedZhang, G, et al. 2025. UBE2D3 functions in mouse oocyte meiotic maturation. FASEB Journal, e70375.
398340925mg/mlLuo, H, et al. 2025. Pathogenic variants of TUBB8 cause oocyte spindle defects by disrupting with EB1/CAKP5 interactions and potential treatment targeting microtubule acetylation through HDAC6 inhibition. Clinical and Translational Medicine, e70193.
397210071:200Zhou, C, et al. 2024. A Microtubule-Associated Protein Functions in Preventing Oocytes from Evading the Spindle Assembly Checkpoint. Advanced Science, e2413097.
394756461:30Biswas, L, et al. 2024. Maternal genetic variants in kinesin motor domains prematurely increase egg aneuploidy. PNAS: USA, e2414963121.
394377841:250Sharma, N, et al. 2024. Changes in DNA repair compartments and cohesin loss promote DNA damage accumulation in aged oocytes. Current Biology, 5131-5148.
393860211:30Blengini, CS, et al. 2024. Genetic interaction mapping of Aurora protein kinases in mouse oocytes. Frontiers in Cell and Developmental Biology, 1455280.
392079141:500Chotiner, JY, et al. 2024. TRIP13 localizes to synapsed chromosomes and functions as a dosage-sensitive regulator of meiosis. eLife, .
392445961:200Ma, A., et al. 2024. FBXO47 regulates centromere pairing as key component of centromeric SCF E3 ligase in mouse spermatocytes. Communications Biology, 1099.
39093409not listedFan, L.H., et al. 2024. MEIKIN expression and its C-terminal phosphorylation by PLK1 is closely related the metaphase-anaphase transition by affecting cyclin B1 and Securin stabilization in meiotic oocyte. Histochemistry and Cell Biology, .
390812931:30Blengini, CS, et al. 2024. Spatio-temporal requirements of Aurora kinase A in mouse oocyte meiotic spindle building. iScience, 110451.
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369754041:20Navarrete-López, P., et al. 2023. An Ancient Testis-Specific IQ Motif-Containing H Gene Regulates Specific Transcript Isoform Expression During Spermatogenesis. Development, dev201334.
368137651:300Zeng, J., et al. 2023. PAK2 is Essential for Chromosome Alignment in Metaphase I Oocytes. Cell Death & Disease, 150.
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368137651:300Zeng, J., et al. 2023. PAK2 is essential for chromosome alignment in metaphase I oocytes. Cell Death and Disease, 150.
368004301:25Kincade, J.N., et al. 2023. Initial spindle positioning at the oocyte center protects against incorrect kinetochore-microtubule attachment and aneuploidy in mice. Science Advances, eadd7397.
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365648611:100Lee, C., et al. 2022. Selective utilization of non‐homologous end‐joining and homologous recombination for DNA repair during meiotic maturation in mouse oocytes. Cell Proliferation, e13384.
365336781:500Jin, Z., et al. 2022. ZP3 and AIPL1 Participate in GVBD of Mouse Oocytes by Affecting the Nuclear Membrane Localization and Maturation of Farnesylated Prelamin A. Zygote, 1-9.
365136381:100Zhou, C.J., et al. 2022. CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I. Nature Communications, 7732.
365039871:300Zhou, Q., et al. 2022. Mitochondrial E3 Ubiquitin Ligase MARCH5 is Required for Mouse Oocyte Meiotic Maturation. Biology of Reproduction, .
364804831:50Zhang, Q., et al. 2022. Supplementation of Mitochondria from Endometrial Mesenchymal Stem Cells Improves Oocyte Quality in Aged Mice. Cell Proliferation, e13372.
363542071:50Chen, L., et al. 2022. Septin 9 controls CCNB1 stabilization via APC/CCDC20 during meiotic metaphase I/anaphase I transition in mouse oocytes. Cell Proliferation, e13359.
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360495891:50Chang, H., et al. 2022. Octocrylene Exposure Impairs Mouse Oocyte Quality by Inducing Spindle Defects and Mitochondria Dysfunction. Toxicology, 153306.
35858239not listedWang, Y., et al. 2022. FBXW24 Controls Female Meiotic Prophase Progression by Regulating SYCP3 Ubiquitination. Clinical and Translational Medicine, e891.
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358103271:200Suebthawinkul, C., et al. 2022. Quantitative Morphokinetic Parameters Identify Novel Dynamics of Oocyte Meiotic Maturation and Cumulus Expansion. Biology of Reproduction, 1097-1112.
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Immunocytochemistry: Porcine
PMID Dilution Publication
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355973241:150Pan, C., et al. 2022. Mogroside V ameliorates the oxidative stress-induced meiotic defects in porcine oocytes in vitro. Reproductive toxicology, .
Immunocytochemistry: Rat
PMID Dilution Publication
346687191:25Begley, M.A., et al. 2021. K-fiber bundles in the mitotic spindle are mechanically reinforced by Kif15. Molecular Biology of the Cell, br11.
Immunocytochemistry: Snake
PMID Dilution Publication
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Immunohistochemistry: Bird
PMID Dilution Publication
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Immunohistochemistry: Bovine
PMID Dilution Publication
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Immunohistochemistry: Fish
PMID Dilution Publication
387957381:100Lisachov, A, et al. 2024. Genome-wide sequence divergence of satellite DNA could underlie meiotic failure in male hybrids of bighead catfish and North African catfish (Clarias, Clariidae). Genomics, 110868.
Immunohistochemistry: Human
PMID Dilution Publication
390021331:500Hsu, J., et al. 2024. Protocol for iterative indirect immunofluorescence imaging in cultured cells, tissue sections, and metaphase chromosome spreads. STAR Protocols, 103190.
367326331:250Harasimov, K., et al. 2023. Actin-driven Chromosome Clustering Facilitates Fast and Complete Chromosome Capture in Mammalian Oocytes. Nature Cell Biology, 439-452.
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339642101:250Cavazza, T., et al. 2021. Parental genome unification is highly error-prone in mammalian embryos. Cell, 2860-2877.
Immunohistochemistry: Mouse
PMID Dilution Publication
398248111:50-1:100Kang, Z, et al. 2025. NKAPL facilitates transcription pause-release and bridges elongation to initiation during meiosis exit. Nature Communications, 791.
39019259not listedJin, Y., et al. 2024. MIB2 functions in oocyte meiosis by modulating chromatin configuration. Molecular & Cellular Proteomics, 100813.
390021331:500Hsu, J, et al. 2024. Protocol for iterative indirect immunofluorescence imaging in cultured cells, tissue sections, and metaphase chromosome spreads. STAR Protocols, 103190.
389602341:50Chang, H., et al. 2024. Fbxo28 is essential for spindle migration and morphology during mouse oocyte meiosis I. International Journal of Biological Macromolecules, 133232.
385537281:100Ju, JQ, et al. 2024. Kinesin KIFC3 is essential for microtubule stability and cytokinesis in oocyte meiosis. Cell Communication and Signaling, 199.
385460431:50Sun, SM, et al. 2024. Loss of UBE2S causes meiosis I arrest with normal spindle assembly checkpoint dynamics in mouse oocytes. Development, 0.
38244729not listedZhang, M., et al. 2024. Prothioconazole exposure disrupts oocyte maturation and fertilization by inducing mitochondrial dysfunction and apoptosis in mice. Free Radical Biology & Medicine, 274-284.
382185861:100Zhang, M., et al. 2024. Copper oxide nanoparticles impairs oocyte meiosis maturation by inducing mitochondrial dysfunction and oxidative stress. Food and Chemical Toxicology, 114441.
379215591:300Zhou, X, et al. 2023. ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progression. Cell proliferation, e13567.
378382131:100Wang, W, et al. 2023. Alpha-lipoic acid supplementation reverses the declining quality of oocytes exposed to cyclophosphamide. Food and Chemical Toxicology, 114090.
37837697not listedZhang, Y, et al. 2023. Butylparaben weakens female fertility via causing oocyte meiotic arrest and fertilization failure in mice. Ecotoxicology and Environmental Safety, 115561.
374811221:150Zhang, H, et al. 2023. LSM14B Coordinates Protein Component Expression in the P-body and Controls Oocyte Maturation. Journal of genetics and genomics = Yi chuan xue bao, 0.
373865161:200Luo, YY, et al. 2023. Growth Hormone Reduces Aneuploidy and Improves Oocytes Quality by JAK2-MAPK3/1 Pathway in Aged Mice. Journal of translational medicine, 426.
373059661:500Cao, G, et al. 2023. PARP12 Regulates Mouse Oocyte Meiotic Maturation. Journal of Cellular Physiology, 1580-1591.
372393821:100Bikchurina, T, et al. 2023. Chromosome Asynapsis Is the Main Cause of Male Sterility in the Interspecies Hybrids of East Asian Voles (Alexandromys, Rodentia, Arvicolinae). Genes, 1022.
370124511:200Suebthawinkul, C., et al. 2023. Morphokinetic Parameters of Mouse Oocyte Meiotic Maturation and Cumulus Expansion are not Affected by Reproductive Age or Ploidy Status. Journal of Assisted Reproduction and Genetics, .
369897151:200Qu, J., et al. 2023. Near-infrared Fluorophore IR-61 Improves the Quality of Oocytes in Aged Mice via Mitochondrial Protection. Biomedicine & Pharmacotherapy, 114571.
Immunohistochemistry: Non-Human Primate
PMID Dilution Publication
347043421:30Blengini, C.S., et al. 2021. Age‐dependent integrity of the meiotic spindle assembly checkpoint in females requires Aurora kinase B. Aging Cell, e13489.
Immunohistochemistry: Porcine
PMID Dilution Publication
367326331:250Harasimov, K., et al. 2023. Actin-driven Chromosome Clustering Facilitates Fast and Complete Chromosome Capture in Mammalian Oocytes. Nature Cell Biology, 439-452.
Immunohistochemistry: Rat
PMID Dilution Publication
390021331:500Hsu, J, et al. 2024. Protocol for iterative indirect immunofluorescence imaging in cultured cells, tissue sections, and metaphase chromosome spreads. STAR Protocols, 103190.
Western Blot: Human
PMID Dilution Publication
40010343not listedMusiani, D, et al. 2025. Uracil processing by SMUG1 in the absence of UNG triggers homologous recombination and selectively kills BRCA1/2-deficient tumors. Molecular Cell, .

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